anti-Toutes les espèces alpha Tubulin anticorps pour Immunohistochemistry (Paraffin-embedded Sections)

Recommended alpha Tubulin Antibody

Antigène
alpha Tubulin (TUBA1) Anticorps
  • Tuba1
  • K-ALPHA-1
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • tuba1b
  • Tuba1b
Reactivité
Toutes les espèces
298
189
178
41
36
35
26
19
17
17
13
10
10
10
8
8
8
8
8
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Hôte
Souris
184
133
24
5
2
2
2
1
Clonalité
Monoclonal
Conjugué
Cet anticorp alpha Tubulin est conjugé à/à la FITC
22
10
8
8
8
8
8
8
7
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
266
111
103
85
72
65
55
31
18
16
13
8
3
2
1
1
1
Options

Images

N° du produit ABIN93892
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
45.156593 ABIN301997 ICC IHC (p) WB Biotin Mouse IgG1 TU-01 13
42.156593 ABIN93891 ICC IHC (p) WB Mouse IgG1 TU-01 22
21.156593 ABIN93894 ICC IHC (p) IP ELISA WB Mouse IgM TU-16 3
11.601543 ABIN239814 Inhibition ICC IHC IHC (p) WB Mouse IgG1 TU-01 0
11.601543 ABIN239816 Inhibition ELISA ICC IHC IHC (p) IP WB Mouse IgM TU-16 0
4 ABIN1724147 ICC IHC (p) WB Mouse IgG1 TU-01 0
4 ABIN1724162 ICC IHC (p) IP ELISA WB Mouse IgM TU-16 0
1 ABIN3199315 ELISA FACS ICC IF IHC (p) IP WB Mouse IgG1 TU-01 0
1 ABIN3199317 ICC IF IHC (p) WB FITC Mouse IgG1 TU-01 0
1 ABIN3199316 ICC IHC (p) WB Biotin Mouse IgG1 TU-01 0
1 ABIN3199319 ELISA ICC IHC (p) IP WB Mouse IgM TU-16 0

General

Antigène alpha Tubulin (TUBA1) Anticorps
Reactivité Toutes les espèces
(298), (189), (178), (41), (36), (35), (26), (19), (17), (17), (13), (10), (10), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Hôte Souris
(184), (133), (24), (5), (2), (2), (2), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp alpha Tubulin est conjugé à/à la FITC
(22), (10), (8), (8), (8), (8), (8), (8), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(266), (111), (103), (85), (72), (65), (55), (31), (18), (16), (13), (8), (3), (2), (1), (1), (1)
Pubmed 14 références disponible
Fournisseur

Détail du produit anti-alpha Tubulin anticorps

Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN93892) Images
Specificité The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Réactivité croisée (Details) Broad species reactivity
Immunogène Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Clone TU-01
Isotype IgG1
Plasmids, Primers & others

Détail du antigène alpha Tubulin

Détail du produit anti-alpha Tubulin anticorps Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN93892) Images Haut de la page
Antigène
Autre désignation alpha-tubulin (TUBA1 Antibody Extrait)
Sujet The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Pathways Dynamique des Microtubules

Information d'application

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Stockage References for anti-alpha Tubulin antibody (ABIN93892) Images Haut de la page
Indications d'application The reagent is designed for direct immunofluorescence analysis on fixed and permeabilized cells. Suggested working dilution is 1:50. It is recommended that the user titrates the reagent for use in the particular testing system.
Commentaires

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only

Stockage

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application References for anti-alpha Tubulin antibody (ABIN93892) Images Haut de la page
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-alpha Tubulin antibody (ABIN93892)

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage Images Haut de la page
Background publications

Miyajima, Maruyama, Nonomura, Hatakeyama: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." dans: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009

Honys, R?nuak, Fecikovua, Jedelskuy, Nebesuarovua, Dobrev, Capkovua: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." dans: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009

Bon, Di Carlo, Folgiero, Avetrani, Lazzari, DOrazi, Brizzi, Sacchi, Soddu, Blandino, Mottolese, Falcioni: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." dans: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009

Produit citée dans:

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." dans: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." dans: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." dans: Planta, Vol. 201, Issue 3, pp. 349-58, 1997

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." dans: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." dans: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." dans: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, 1993

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." dans: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." dans: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." dans: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." dans: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, 1987

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." dans: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, 1982

Images

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN93892) Haut de la page
Images (Fournisseur)
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-al...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Nucleus is stained with DAPI (blue).
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Flow Cytometry (FACS) image for anti-alpha Tubulin (TUBA1) antibody (FITC) (ABIN93892) Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal an...