anti-Invertébré alpha Tubulin anticorps pour ELISA

Recommended alpha Tubulin Antibody

Antigène
alpha Tubulin (TUBA1) Anticorps
  • Tuba1
  • K-ALPHA-1
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • tuba1b
  • Tuba1b
Épitope
AA 419-435
32
28
17
12
11
9
9
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Invertébré, Souris, Rat (Rattus), Vertebrate, Yeast (Saccharomyces cerevisiae)
295
186
175
41
36
35
25
19
18
17
13
10
10
10
8
8
8
8
8
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Hôte
Souris
184
131
24
5
2
2
2
1
Clonalité
Monoclonal
Conjugué
Cet anticorp alpha Tubulin est non-conjugé
22
10
8
8
8
8
8
8
8
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
264
110
103
85
73
65
54
31
18
16
13
8
3
2
1
1
1
Options

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N° du produit ABIN1742547
$ 478.56
Plus shipping costs $45.00

General

Antigène alpha Tubulin (TUBA1) Anticorps
Épitope AA 419-435
(32), (28), (17), (12), (11), (9), (9), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Invertébré, Souris, Rat (Rattus), Vertebrate, Yeast (Saccharomyces cerevisiae)
(295), (186), (175), (41), (36), (35), (25), (19), (18), (17), (13), (10), (10), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Souris
(184), (131), (24), (5), (2), (2), (2), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp alpha Tubulin est non-conjugé
(22), (10), (8), (8), (8), (8), (8), (8), (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(264), (110), (103), (85), (73), (65), (54), (31), (18), (16), (13), (8), (3), (2), (1), (1), (1)
Pubmed 3 références disponible
Fournisseur

Détail du produit anti-alpha Tubulin anticorps

Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN1742547) Images
Specificité Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
Purification purified IgG. Azide was added before lyophilization.
Immunogène Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Clone 3A2
Isotype IgG1
Plasmids, Primers & others

Détail du antigène alpha Tubulin

Détail du produit anti-alpha Tubulin anticorps Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN1742547) Images Haut de la page
Antigène
Autre désignation alpha-Tubulin (TUBA1 Antibody Extrait)
Pathways Dynamique des Microtubules

Information d'application

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Stockage References for anti-alpha Tubulin antibody (ABIN1742547) Images Haut de la page
Indications d'application WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200
Commentaires

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Restrictions For Research Use only

Stockage

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application References for anti-alpha Tubulin antibody (ABIN1742547) Images Haut de la page
Format Lyophilized
Reconstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Stock -20 °C
Stockage commentaire Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-alpha Tubulin antibody (ABIN1742547)

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage Images Haut de la page
Produit citée dans:

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." dans: Frontiers in cellular neuroscience, Vol. 9, pp. 24, 2015

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." dans: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." dans: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, 2013

Images

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN1742547) Haut de la page
Images (Fournisseur)
Immunocytochemistry (ICC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) anti-alpha Tubulin (TUBA1) (AA 419-435) antibody
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). N...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) (AA 419-435) antibody (ABIN1742547) dilution: 1 : 1000, sample: rat brain homogenate