anti-Humain SPRY1 anticorps pour Flow Cytometry

Recommended SPRY1 Antibody

Antigène
Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) Anticorps
  • hSPRY1
  • SPRY1
  • LOC100223875
  • hspry1
  • xspry1
  • sprouty
  • sprouty1
  • sprouty-1
  • Xtsprouty1
  • spry1a
  • spry1b
  • sprouty RTK signaling antagonist 1
  • sprouty RTK signaling antagonist 1 L homeolog
  • sprouty RTK signaling antagonist 1 S homeolog
  • sprouty homolog 1 (Drosophila)
  • SPRY1
  • Spry1
  • spry1
  • spry1.L
  • spry1.S
Épitope
C-Term
11
8
7
6
5
5
4
3
1
1
1
Reactivité
Humain, Souris
54
19
4
3
3
3
2
2
2
2
1
1
1
1
Hôte
Chèvre
34
15
13
2
Clonalité
Polyclonal
Conjugué
Cet anticorp SPRY1 est non-conjugé
4
4
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
58
31
24
8
5
3
3
3
2
1
1
1
1
Options

Images

N° du produit ABIN570913
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
18.340187 ABIN5932371 ELISA FACS IF/ICC IHC WB Goat Polyclonal 0
10 ABIN4952690 ELISA FACS ICC IF IHC (p) WB Goat Ig Fraction Polyclonal 0

General

Antigène Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) Anticorps
Épitope C-Term
(11), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
Reactivité Humain, Souris
(54), (19), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Hôte Chèvre
(34), (15), (13), (2)
Clonalité
Polyclonal
Conjugué Cet anticorp SPRY1 est non-conjugé
(4), (4), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(58), (31), (24), (8), (5), (3), (3), (3), (2), (1), (1), (1), (1)
Fournisseur

Détail du produit anti-SPRY1 anticorps

Détail du antigène SPRY1 Information d'application Stockage Images
Fonction Sprouty
Blocking Peptide Blocking peptide for this product available: ABIN2816486
Séquence CPSRGQGKPS
Specificité NP_005832.1 and NP_955359.1 represent varients of the same protein.
Homologie Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.
Plasmids, Primers & others

Détail du antigène SPRY1

Détail du produit anti-SPRY1 anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation SPRY1 (SPRY1 Antibody Extrait)
Sujet SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
ID gène 10252
NCBI Accession NP_005832, NP_955359
Pathways EGFR Signaling Pathway, EGFR Downregulation

Information d'application

Détail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Stockage Images Haut de la page
Indications d'application

DS WB Results: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3 μg/mL.

DS IHC Results: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Information d'application Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

Images

Détail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) (C-Term) antibody (ABIN570913) ABIN570913 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). ...
Immunofluorescence (IF) image for anti-Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) (C-Term) antibody (ABIN570913) ABIN570913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabi...
Flow Cytometry (FACS) image for anti-Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) (C-Term) antibody (ABIN570913) ABIN570913 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line...