CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays

Détails pour le produit réf. ABIN6950951
Antigène
Hôte
Escherichia coli (E. coli)
Application
Chromatin Immunocleavage (ChIC), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
Options
Fonction This construct of Protein A and G fused to Micrococcal Nuclease is useful in performing CUT&RUN.

The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.

Marque CUTANA™
Attributs du produit Recombinantly produced in E. coli, CUTANA™ pAGMNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA pAG-MNase contains a C-terminal 6xHis epitope tag.
Antigène
Poids moléculaire 44 kDa
Indications d'application This product is sufficient to perform 50/250 CUT&RUN reactions.
Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&RUN reaction (20X dilution).
Commentaires

CUTANA pAG-MNase, the essential reagent for ChIC/CUT&RUN workflows:

  • First-in-class commercial product for ChIC/CUT&RUN assays
  • Optimized fusion of Proteins A and G with Micrococcal Nuclease (pAG-MNase) enables direct compatibility with a broad range of antibody isotypes
  • 50 and 250 reaction pack sizes, enabling greater experimental throughput

Restrictions For Research Use only
Concentration 20 X
Buffer Provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50 % glycerol.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -20 °C
Stockage commentaire Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951) A representative 350 kb region of an H3K4me1 profile in K-562 cells, generated using ...
Image no. 2 for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951) Size Distribution of Released Chromatin:CUT&RUN was performed using CUTANA™ pAG-MNase...
Image no. 3 for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951) Protein Gel Data: CUTANA™ pAG-MNase (1 μg) was resolved via SDS-PAGE and stained with...
Image no. 4 for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951) CUT&RUN Data:Sequencing tracks obtained using CUTANA™ pAG-MNase. (A) CUT&RUN was perf...
Image no. 5 for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951) Summary of the CUT&RUN protocol using a primary and secondary antibody (left). Subseq...
Produit citée dans: Meers, Bryson, Henikoff, Henikoff: "Improved CUT&RUN chromatin profiling tools." dans: eLife, Vol. 8, 2019 (PubMed).

Skene, Henikoff, Henikoff: "Targeted in situ genome-wide profiling with high efficiency for low cell numbers." dans: Nature protocols, Vol. 13, Issue 5, pp. 1006-1019, 2019 (PubMed).

Skene, Henikoff: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites." dans: eLife, Vol. 6, 2018 (PubMed).

Schmid, Durussel, Laemmli: "ChIC and ChEC; genomic mapping of chromatin proteins." dans: Molecular cell, Vol. 16, Issue 1, pp. 147-57, 2004 (PubMed).