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CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays Enzyme

ChIC, CUT&RUN This construct of Protein A and G fused to Micrococcal Nuclease is useful in performing CUT&RUN.

The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.

Pubmed (4)
N° du produit ABIN6950951
$330.00
Plus shipping costs $45.00
50 reactions ABIN6950951
50 reactions ABIN6950951
local_shipping Destination: Etats-Unis
Envoi sous 3 à 6 jours ouvrables
  • Antigène
    Hôte
    Escherichia coli (E. coli)
    Application
    Chromatin Immunocleavage (ChIC), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Fonction
    This construct of Protein A and G fused to Micrococcal Nuclease is useful in performing CUT&RUN.

    The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.

    Marque
    CUTANA™
    Attributs du produit
    Recombinantly produced in E. coli, CUTANA™ pAGMNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA pAG-MNase contains a C-terminal 6xHis epitope tag.
  • Indications d'application
    This product is sufficient to perform 50/250 CUT&RUN reactions.
    Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&RUN reaction (20X dilution).
    Commentaires

    CUTANA pAG-MNase, the essential reagent for ChIC/CUT&RUN workflows:

    • First-in-class commercial product for ChIC/CUT&RUN assays
    • Optimized fusion of Proteins A and G with Micrococcal Nuclease (pAG-MNase) enables direct compatibility with a broad range of antibody isotypes
    • 50 and 250 reaction pack sizes, enabling greater experimental throughput

    Restrictions
    For Research Use only
  • Concentration
    20 X
    Buffer
    Provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50 % glycerol.
    Conseil sur la manipulation
    Avoid freeze-thaw cycles
    Stock
    -20 °C
    Stockage commentaire
    Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
    Date de péremption
    12 months
  • Meers, Bryson, Henikoff, Henikoff: "Improved CUT&RUN chromatin profiling tools." dans: eLife, Vol. 8, 2019 (PubMed).

    Skene, Henikoff, Henikoff: "Targeted in situ genome-wide profiling with high efficiency for low cell numbers." dans: Nature protocols, Vol. 13, Issue 5, pp. 1006-1019, 2019 (PubMed).

    Skene, Henikoff: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites." dans: eLife, Vol. 6, 2018 (PubMed).

    Schmid, Durussel, Laemmli: "ChIC and ChEC; genomic mapping of chromatin proteins." dans: Molecular cell, Vol. 16, Issue 1, pp. 147-57, 2004 (PubMed).

  • Antigène
    Poids moléculaire
    44 kDa
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