Caspase-3 Inhibitor Z-DEVD-FMK Inhibitor
- Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
- A caspase-3 inhibitor
- Attributs du produit
- PubChem CID: 16760394
A synthetic peptide that irreversibly inhibits activity of DEVD-dependent caspases (e.g., caspase-3 and caspase-7) and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
- > 98 % by HPLC
- Chemical Name
- Z-DEVD-FMK, Caspase-3 inhibitor, Z-DEVD fluoromethylketone
InhA 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamateGeldanamycin- FITC Inhibitor
InhA 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate
- Indications d'application
- We recommend using 1000-5000X dilutions for inhibiting caspase-3 activity in Jurkat cell culture (e.g., Add 1 µL to 1-5 mL of culture medium). The inhibitor can be added t°Culture medium together with apoptosis inducer or 1 hr before inducer is added. The optimal doses may vary for different cells and culture conditions.
- For Research Use only
- 10 mM
- Conseil sur la manipulation
- Protect from light and moisture
- -20 °C
- Date de péremption
- 6-12 months
Kim, Zhang, Hitomi, Lee, Mukherjee: "Endoplasmic reticulum stress-induced caspase-4 activation mediates apoptosis and neurodegeneration in INCL." dans: Human molecular genetics, Vol. 15, Issue 11, pp. 1826-34, 2006 (PubMed).
Lin, Cheung, Wu, Chen, Lin, Chen, Liou, Shyue, Wu: "15d-prostaglandin J2 protects brain from ischemia-reperfusion injury." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 481-7, 2006 (PubMed).
Ma, Li, Chiu, Wang, Sloane, Lü, Kosaras, Sidman, Volpe, Vartanian: "Toll-like receptor 8 functions as a negative regulator of neurite outgrowth and inducer of neuronal apoptosis." dans: The Journal of cell biology, Vol. 175, Issue 2, pp. 209-15, 2006 (PubMed).
Mao, Iwai, Keng, Vulapalli, Liang: "Norepinephrine-induced oxidative stress causes PC-12 cell apoptosis by both endoplasmic reticulum stress and mitochondrial intrinsic pathway: inhibition of phosphatidylinositol 3-kinase survival pathway." dans: American journal of physiology. Cell physiology, Vol. 290, Issue 5, pp. C1373-84, 2006 (PubMed).
Nair, McNaught, González-Maeso, Sealfon, Olanow: "p53 mediates nontranscriptional cell death in dopaminergic cells in response to proteasome inhibition." dans: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39550-60, 2006 (PubMed).
Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." dans: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).
Kubota, Lee, Tsuchiya, Young, Everett, Martinez-Mier, Snead, Nguyen, Urano, Bartlett: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." dans: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).
Bi, Stuelten, Kilts, Wadhwa, Iozzo, Robey, Chen, Young: "Extracellular matrix proteoglycans control the fate of bone marrow stromal cells." dans: The Journal of biological chemistry, Vol. 280, Issue 34, pp. 30481-9, 2005 (PubMed).
Miura, Chen, Allen, Bi, Gronthos, Seo, Lakhani, Flavell, Feng, Robey, Young, Shi: "A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells." dans: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1704-13, 2004 (PubMed).
Song, Mahidhara, Zhou, Hoffman, Seol, Flavell, Billiar, Otterbein, Choi: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).
Song, Zhou, Kim, Shapiro, Liu, Ferran, Choi, Otterbein: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." dans: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).
Wang, Grégori, Hullinger, Andrisani et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor ..." dans: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).
Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." dans: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).
- Kim, Zhang, Hitomi, Lee, Mukherjee: "Endoplasmic reticulum stress-induced caspase-4 activation mediates apoptosis and neurodegeneration in INCL." dans: Human molecular genetics, Vol. 15, Issue 11, pp. 1826-34, 2006 (PubMed).
- Poids moléculaire
- 668.7 g/mol