anti-Singe 5-Hydroxytryptamine (serotonin) Receptor 2C anticorps pour Immunohistochemistry (Paraffin-embedded Sections)

Recommended 5-Hydroxytryptamine (serotonin) Receptor 2C Antibody (fourni par: Connectez-vous pour afficher )

Antigène
5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) Anticorps
  • 5-HT2C
  • 5-HTR2C
  • HTR1C
  • 5-HT2cR
  • 5HT1c
  • Htr1c
  • SR1
  • 5HT-1C
  • 5HT2C
  • 5-HT-2C
  • 5-ht2cr
  • si:dkey-24g18.2
  • 5ht2c
  • 5HTR2C
  • 5-hydroxytryptamine receptor 2C
  • 5-hydroxytryptamine (serotonin) receptor 2C
  • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog
  • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1
  • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
  • HTR2C
  • Htr2c
  • htr2c.S
  • htr2cl1
  • LOC407134
Épitope
Cytoplasmic Domain
20
11
11
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
Reactivité
Humain, Singe
143
56
54
9
5
3
3
3
3
2
Hôte
Lapin
114
17
17
Clonalité
Polyclonal
Conjugué
Inconjugué
5
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
98
56
34
31
13
11
10
6
6
3
1
1
1
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN1048270
$ 484.00
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General

Antigène 5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) Anticorps
Épitope Cytoplasmic Domain
(20), (11), (11), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivité Humain, Singe
(143), (56), (54), (9), (5), (3), (3), (3), (3), (2)
Hôte Lapin
(114), (17), (17)
Clonalité
Polyclonal
Conjugué Inconjugué
(5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(98), (56), (34), (31), (13), (11), (10), (6), (6), (3), (1), (1), (1)
Fournisseur Connectez-vous pour afficher

Détail du produit

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Marque IHC-plus™
Specificité Human 5HT2C Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant (100%) Horse, Pig (94%) Bat, Panda, Rabbit (81%).
Purification Immunoaffinity purified
Immunogène Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT2C Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant (100%), Horse, Pig (94%), Bat, Panda, Rabbit (81%).

Type of Immunogen: Synthetic peptide

Détail du antigène

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Antigène
Autre désignation HTR2C / 5-HT2C Receptor (HTR2C Antibody Extrait)
Sujet Name/Gene ID: HTR2C
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR2C, 5-HT-1C, 5-HT1c receptor, 5HT-1C, 5-HT2C, 5-HT2c receptor, 5HT2C Receptor, 5-HT-2C, 5-HT1C, 5-HTR2C, HTR1C, Serotonin 5-HT-1c receptor, Serotonin receptor 2C, Serotonin 1c receptor, Serotonin 5-HT-2C receptor, Serotonin 2c receptor
ID gène 3358
Pathways Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour

Information d'application

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Indications d'application Approved: IHC, IHC-P (2.6 μg/mL)
Commentaires

Target Species of Antibody: Human

Procédure de l'essai The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Stockage

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months

Images

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Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) (Cytoplasmic Domain) antibody (ABIN1048270) Anti-5HT2C Receptor antibody ABIN1048270 IHC staining of human pancreas, ganglion an...