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Souris IgM isotype control (PE)

IsoC, FACS, IF IgM PE
N° du produit ABIN2145411
  • Antigène Tous les produits IgM
    IgM
    Hôte
    • 62
    • 37
    • 37
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Souris
    Conjugué
    PE
    Application
    Isotype Control (IsoC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Attributs du produit
    This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
    Isotype
    IgM
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Procédure de l'essai
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Antigène
    IgM
    Abstract
    IgM Produits
    Synonymes
    IgM constant region Isotype Control, IGM Isotype Control
    Classe de substances
    Antibody
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