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HEK-293 Cells IgG2a Isotype Control

IsoC IgG2a unconjugated
N° du produit ABIN2181277
  • Antigène Tous les produits IgG2a
    IgG2a
    Reactivité
    Souris
    Hôte
    • 183
    • 75
    • 2
    • 1
    HEK-293 Cells
    Application
    Isotype Control (IsoC)
    Attributs du produit
    This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa under reducing (R) condition, and 50-60 kDa under non-reducing (NR) condition (SDS-PAGE).
    Pureté
    >90 % as determined by SDS-PAGE.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
    Isotype
    IgG2a
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    Buffer
    50 mM Tris, 100 mM Glycine, pH 7.5
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Synonymes
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Classe de substances
    Antibody
    Sujet
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
    Poids moléculaire
    26.4 kDa
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