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CYP7B1 Kit ELISA

CYP7B1 Reactivité: Humain Colorimetric Sandwich ELISA
N° du produit ABIN1118773
  • Antigène Voir toutes CYP7B1 Kits ELISA
    CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of human 25-hydroxycholesterol 7-alpha-hydroxylase,Oxysterol 7-alpha-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and other biological fluids.
    Analytical Method
    Quantitative
    Ingrédients
    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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  • Commentaires

    Gene Name: CYP7B1

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxysterol 7-alpha-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Oxysterol 7-alpha-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Oxysterol 7-alpha-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Oxysterol 7-alpha-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit, Substrate should be always stored at 4°C. Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C(Except the substrate). The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
  • Antigène Voir toutes CYP7B1 Kits ELISA
    CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))
    Autre désignation
    25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1 Produits)
    Synonymes
    CYP7B1 Kit ELISA, CBAS3 Kit ELISA, CP7B Kit ELISA, SPG5A Kit ELISA, AW261589 Kit ELISA, D3Ertd552e Kit ELISA, hct-1 Kit ELISA, cytochrome P450 family 7 subfamily B member 1 Kit ELISA, cytochrome P450, family 7, subfamily B, polypeptide 1 Kit ELISA, cytochrome P450, family 7, subfamily b, polypeptide 1 Kit ELISA, CYP7B1 Kit ELISA, cyp7b1 Kit ELISA, Cyp7b1 Kit ELISA
    Sujet
    Synonyms: 25-hydroxycholesterol 7-alpha-hydroxylase,CYP7B1,Cytochrome P450 7B1,Homo sapiens,Human,Oxysterol 7-alpha-hydroxylase
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling
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