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ATG7 Kit ELISA

ATG7 Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN1568538
  • Antigène Voir toutes ATG7 Kits ELISA
    ATG7 (ATG7 Autophagy Related 7 (ATG7))
    Reactivité
    • 2
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATG7 in mouse tissue homogenates, cell lysates and other biological fluids.
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    0.053 ng/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Procédure de l'essai

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes ATG7 Kits ELISA
    ATG7 (ATG7 Autophagy Related 7 (ATG7))
    Autre désignation
    ATG7 (ATG7 Produits)
    Synonymes
    APG7-LIKE Kit ELISA, APG7L Kit ELISA, GSA7 Kit ELISA, D8 Kit ELISA, UBA1B Kit ELISA, UBE1L Kit ELISA, UBE2 Kit ELISA, Ube1l Kit ELISA, 1300004C08Rik Kit ELISA, AO090001000744 Kit ELISA, atg7 Kit ELISA, 1810013K23Rik Kit ELISA, Agp7 Kit ELISA, Apg7l Kit ELISA, Atg7l Kit ELISA, ATAPG7 Kit ELISA, ATATG7 Kit ELISA, ATG7 Kit ELISA, AUTOPHAGY 7 Kit ELISA, AUTOPHAGY-RELATED 7 Kit ELISA, K15I22.10 Kit ELISA, K15I22_10 Kit ELISA, autophagy related 7 Kit ELISA, ubiquitin like modifier activating enzyme 7 Kit ELISA, ubiquitin-like modifier activating enzyme 7 Kit ELISA, autophagy-related protein 7 Kit ELISA, Autophagy-related protein 7 Kit ELISA, Apg7p Kit ELISA, ThiF family protein Kit ELISA, ubiquitin-like conjugating enzyme Atg7 Kit ELISA, ATG7 Kit ELISA, UBA7 Kit ELISA, Uba7 Kit ELISA, ANI_1_3368024 Kit ELISA, AOR_1_1338164 Kit ELISA, PTRG_02236 Kit ELISA, PAAG_00287 Kit ELISA, PITG_12537 Kit ELISA, atg7 Kit ELISA, Atg7 Kit ELISA, APG7 Kit ELISA
    Sujet
    Alternative name: APG7L, GSA7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Ubiquitin-activating enzyme E1-like protein
    ID gène
    74244
    UniProt
    Q9D906
    Pathways
    Response to Water Deprivation, Autophagy
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