CYP1A1 Kit ELISA

N° du produit ABIN1569308

Détail du produit CYP1A1 Kit ELISA

Antigène: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYP1A1 in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.129 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Autre désignation: CYP1A1 (CYP1A1 Produits)

Synonymes: AHH Kit ELISA, AHRR Kit ELISA, CP11 Kit ELISA, CYP1 Kit ELISA, P1-450 Kit ELISA, P450-C Kit ELISA, P450DX Kit ELISA, Cyp1a2 Kit ELISA, P450-1 Kit ELISA, cyp1a1 Kit ELISA, CYP1A1 Kit ELISA, CYPIA1 Kit ELISA, Cyp45c Kit ELISA, Cypc45c Kit ELISA, P-450MC Kit ELISA, wu:fb63b04 Kit ELISA, zfCYP1A Kit ELISA, zgc:109747 Kit ELISA, ahh Kit ELISA, ahrr Kit ELISA, cp11 Kit ELISA, cyp1 Kit ELISA, cyp1a Kit ELISA, p1-450 Kit ELISA, p450-c Kit ELISA, p450dx Kit ELISA, cytochrome P450 family 1 subfamily A member 1 Kit ELISA, cytochrome P450 1A1 Kit ELISA, cytochrome P450, family 1, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 1, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450 family 1 subfamily D polypeptide 1 Kit ELISA, cytochrome P4501A1 Kit ELISA, polycyclic hydrocarbon-inducible cytochrome P450c Kit ELISA, cytochrome P450, family 1, subfamily A Kit ELISA, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 Kit ELISA, cytochrome P450 family 1 subfamily A member 1 L homeolog Kit ELISA, CYP1A1 Kit ELISA, CpipJ_CPIJ010542 Kit ELISA, Cyp1a1 Kit ELISA, cyp1d1 Kit ELISA, LOC100328613 Kit ELISA, cyp1a Kit ELISA, LOC102129994 Kit ELISA, cyp1a1.L Kit ELISA

Sujet: Alternative name: CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

ID gène: 24296

UniProt: P00185

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha