Hexokinase 1 Kit ELISA

N° du produit ABIN1570307

Détail du produit Hexokinase 1 Kit ELISA

Antigène: Hexokinase 1 (HK1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HK1 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 27.2 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: Hexokinase 1 (HK1)

Autre désignation: HK1 (HK1 Produits)

Synonymes: HK1-ta Kit ELISA, HK1-tb Kit ELISA, HK1-tc Kit ELISA, HKD Kit ELISA, HKI Kit ELISA, HMSNR Kit ELISA, HXK1 Kit ELISA, Hexokinase Kit ELISA, hk1 Kit ELISA, HEX Kit ELISA, HEX-1 Kit ELISA, HK Kit ELISA, ARABIDOPSIS THALIANA HEXOKINASE 1 Kit ELISA, ATHXK1 Kit ELISA, F19B15.160 Kit ELISA, F19B15_160 Kit ELISA, GIN2 Kit ELISA, GLUCOSE INSENSITIVE 2 Kit ELISA, HEXOKINASE Kit ELISA, hexokinase 1 Kit ELISA, im:7148527 Kit ELISA, wu:fc09d08 Kit ELISA, wu:fc16e02 Kit ELISA, wu:fc21e02 Kit ELISA, wu:fq14b11 Kit ELISA, zgc:55790 Kit ELISA, zgc:77618 Kit ELISA, hexokinase-1 Kit ELISA, HK1 Kit ELISA, BB404130 Kit ELISA, Hk-1 Kit ELISA, Hk1-s Kit ELISA, dea Kit ELISA, mHk1-s Kit ELISA, HEXOKIN Kit ELISA, Hxk1 Kit ELISA, NtHxK1 Kit ELISA, NtHxk Kit ELISA, hexokinase Kit ELISA, StHK1 Kit ELISA, hxk Kit ELISA, hk1-A Kit ELISA, hexokinase 1 Kit ELISA, hexokinase-1 Kit ELISA, Hexokinase 1 Kit ELISA, hexokinase 1, putative Kit ELISA, hexokinase 1 L homeolog Kit ELISA, HK1 Kit ELISA, hk1 Kit ELISA, LOC100136279 Kit ELISA, Hex1 Kit ELISA, HXK1 Kit ELISA, Hk1 Kit ELISA, TA19800 Kit ELISA, hxk1 Kit ELISA, LOC107797523 Kit ELISA, LOC102604144 Kit ELISA, hk1.L Kit ELISA

Sujet: Alternative name: HK1-Ta, HK1-Tb, HK1-Tc, HKI, HXK1, Brain form hexokinase

ID gène: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, L'effet Warburg