VCP Kit ELISA

N° du produit ABIN1573032

Détail du produit VCP Kit ELISA

Antigène: VCP (Valosin Containing Protein (VCP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of VCP in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.122 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: VCP (Valosin Containing Protein (VCP))

Autre désignation: VCP (VCP Produits)

Synonymes: ALS14 Kit ELISA, IBMPFD Kit ELISA, TERA Kit ELISA, p97 Kit ELISA, CDC48 Kit ELISA, wu:fd16d05 Kit ELISA, wu:fj63d11 Kit ELISA, p93 Kit ELISA, 3110001E05 Kit ELISA, p97/VCP Kit ELISA, 22.26 Kit ELISA, 7.12 Kit ELISA, Apple Kit ELISA, BcDNA.GM02885 Kit ELISA, BcDNA:GM02885 Kit ELISA, CG2331 Kit ELISA, DmTER94 Kit ELISA, Dmel\CG2331 Kit ELISA, I Kit ELISA, Ter94 Kit ELISA, Tera Kit ELISA, VCP Kit ELISA, VCP ATPase Kit ELISA, VCP/p97 Kit ELISA, Vcp Kit ELISA, anon-WO2004063362.65 Kit ELISA, anon-WO2004063362.67 Kit ELISA, cdc48 Kit ELISA, dTERA Kit ELISA, dVCP Kit ELISA, dmTERA Kit ELISA, dvcp Kit ELISA, l(2)03775 Kit ELISA, l(2)46CFf Kit ELISA, l(2)46CFs Kit ELISA, l(2)46Ch Kit ELISA, pTER94 Kit ELISA, ter94 Kit ELISA, vcp Kit ELISA, FANCG Kit ELISA, valosin containing protein Kit ELISA, valosin containing protein S homeolog Kit ELISA, valosin-containing protein Kit ELISA, CG2331 gene product from transcript CG2331-RA Kit ELISA, zgc:136908 Kit ELISA, transitional endoplasmic reticulum ATPase Kit ELISA, VCP Kit ELISA, vcp.S Kit ELISA, vcp Kit ELISA, Vcp Kit ELISA, Bm1_56585 Kit ELISA, LOC100136246 Kit ELISA, TER94 Kit ELISA, zgc:136908 Kit ELISA, LOC101109680 Kit ELISA

Sujet: Alternative name: IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit

ID gène: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway