CXCL1 Kit ELISA
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- Antigène Voir toutes CXCL1 Kits ELISA
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- Épitope
- AA 35-107
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6-1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL1
- Marque
- PicoKine™
- Type d'échantillon
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- Specificité
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Expression system for standard: E.coli
Immunogen sequence: A35-N107 - Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- <1pg/mL
- Matériel non inclus
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogène
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Expression system for standard: E.coli
Immunogen sequence: A35-N107 - Featured
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- Indications d'application
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Commentaires
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
- Plaque
- Pre-coated
- Protocole
- human CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A35-N107) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL1 amount of sample captured in plate.
- Procédure de l'essai
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL1 standard solution and each sample be measured in duplicate.
- Précision du teste
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- Sample 1: n=16, Mean(pg/ml): 83.8, Standard deviation: 3.6, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 357, Standard deviation: 12.8, CV(%): 3.6
- Sample 3: n=16, Mean(pg/ml): 723, Standard deviation: 24.6, CV(%): 3.4,
- Sample 1: n=24, Mean(pg/ml): 95.9, Standard deviation: 5.85, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 363, Standard deviation: 18.9, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 45.82, CV(%): 6.4
- Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Avoid multiple freeze-thaw cycles.
- Stock
- -20 °C,4 °C
- Stockage commentaire
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Date de péremption
- 12 months
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IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." dans: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
: "After insufficient radiofrequency ablation, tumor-associated endothelial cells exhibit enhanced angiogenesis and promote invasiveness of residual hepatocellular carcinoma." dans: Journal of translational medicine, Vol. 10, pp. 230, (2013) (PubMed).
: "
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IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." dans: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
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- Antigène Voir toutes CXCL1 Kits ELISA
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- Autre désignation
- CXCL1 (CXCL1 Produits)
- Synonymes
- FSP Kit ELISA, GRO1 Kit ELISA, GROa Kit ELISA, MGSA Kit ELISA, MGSA-a Kit ELISA, NAP-3 Kit ELISA, SCYB1 Kit ELISA, CINC-1 Kit ELISA, Gro1 Kit ELISA, CXCL1 Kit ELISA, Sdf1 Kit ELISA, Fsp Kit ELISA, KC Kit ELISA, Mgsa Kit ELISA, N51 Kit ELISA, Scyb1 Kit ELISA, gro Kit ELISA, GRO Kit ELISA, Gro Kit ELISA, C-X-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) Kit ELISA, C-X-C motif chemokine ligand 12 Kit ELISA, chemokine (C-X-C motif) ligand 1 Kit ELISA, CXCL1 Kit ELISA, Cxcl1 Kit ELISA, Cxcl12 Kit ELISA, GRO1 Kit ELISA
- Sujet
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Protein Function: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. .
Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Synonyms: Growth-regulated alpha protein,C-X-C motif chemokine 1,GRO-alpha(1-73),Melanoma growth stimulatory activity,MGSA,Neutrophil-activating protein 3,NAP-3,GRO-alpha(4-73),GRO-alpha(5-73),GRO-alpha(6-73),CXCL1,GRO, GRO1, GROA, MGSA, SCYB1,
Full Gene Name: Growth-regulated alpha protein
Cellular Localisation: Secreted. - ID gène
- 2919
- UniProt
- P09341
- Pathways
- Autophagy
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