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SERPINA4 Kit ELISA

SERPINA4 Reactivité: Humain AA 21-427 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672812
  • Antigène Voir toutes SERPINA4 Kits ELISA
    SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
    Épitope
    AA 21-427
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallistatin/Serpina4
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Q21-P427
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Q21-P427
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the serpin family.

    Tissue Specificity: Expressed by the liver and secreted in plasma.

    Plaque
    Pre-coated
    Protocole
    human Kallistatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallistatin has been precoated onto 96-well plates. Standards(NSO, Q21-P427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallistatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallistatin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1521, Standard deviation: 77.6, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 3786, Standard deviation: 170.4, CV(%): 4.5
    • Sample 3: n=16, Mean(pg/ml): 6866, Standard deviation: 425.7, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 4242, Standard deviation: 237.6, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 7128, Standard deviation: 499, CV(%): 7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes SERPINA4 Kits ELISA
    SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
    Autre désignation
    SERPINA4 (SERPINA4 Produits)
    Synonymes
    KLKR Kit ELISA, Klk6 Kit ELISA, hK1 Kit ELISA, KAL Kit ELISA, KLST Kit ELISA, KST Kit ELISA, PI-4 Kit ELISA, PI4 Kit ELISA, kallistatin Kit ELISA, SERPINA4 Kit ELISA, DKFZp469E1737 Kit ELISA, KALps Kit ELISA, Klst Kit ELISA, kallikrein 1 Kit ELISA, serpin family A member 4 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 Kit ELISA, KLK1 Kit ELISA, SERPINA4 Kit ELISA, Serpina4 Kit ELISA, SPIA4 Kit ELISA, Serpina4-ps1 Kit ELISA
    Sujet

    Protein Function: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .

    Background: Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

    Synonyms: Kallistatin,Kallikrein inhibitor,Peptidase inhibitor 4,PI-4,Serpin A4,SERPINA4,KST, PI4,

    Full Gene Name: Kallistatin

    Cellular Localisation: Secreted.
    ID gène
    5267
    UniProt
    P29622
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