RANKL Kit ELISA

N° du produit ABIN1672814

Détail du produit RANKL Kit ELISA

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Épitope: AA 72-316

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFSF11/RANKL

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: R72-D316

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: R72-D316

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Highly expressed in thymus and lymph nodes, but not in non-lymphoid tissues and is abundantly expressed in T- cells but not in B-cells. A high level expression is also seen in the trabecular bone and lung.

Plaque: Pre-coated

Protocole: mouse TNFSF11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF11 has been precoated onto 96-well plates. Standards(NSO, R72-D316) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF11 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFSF11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF11 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 535, Standard deviation: 18.2, CV(%): 3.4
• Sample 2: n=16, Mean(pg/ml): 1352, Standard deviation: 63.6, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 2366, Standard deviation: 132.5, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 609, Standard deviation: 25, CV(%): 4.1
• Sample 2: n=24, Mean(pg/ml): 1233, Standard deviation: 72.75, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 2547, Standard deviation: 163.1, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour RANKL Kit ELISA (ABIN1672814)

Wu, Wu, Li, Cong, Chen, Xu, Biswas, Liu, Xia, Li, Hu, Zhang, Habib, Zhang, Zou, Zhang, Zhang, Li: "Bone Size and Quality Regulation: Concerted Actions of mTOR in Mesenchymal Stromal Cells and Osteoclasts." dans: Stem cell reports, Vol. 8, Issue 6, pp. 1600-1616, (2017) (PubMed).

Guo, Li, Wu, Jing, Huang, Zhang, Xiang, Ren, Lv, Xiao, Guo: "Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR." dans: Frontiers in pharmacology, Vol. 8, pp. 693, (2017) (PubMed).

Zhao, Cai, Wang, Zhao, Li, Liu, Guan, Zhu, Xiao: "Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Activity." dans: Frontiers in pharmacology, Vol. 8, pp. 928, (2017) (PubMed).

Zhang, Guan, Li, Fang, Chen, Li: "Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss." dans: Scientific reports, Vol. 5, pp. 13575, (2016) (PubMed).

Montesi, Panseri, Iafisco, Adamiano, Tampieri: "Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis." dans: PLoS ONE, Vol. 10, Issue 7, pp. e0132633, (2015) (PubMed).

Zhao, Ning, Wang, Li, Qiao, Yao, Qin: "Calcitonin gene-related peptide inhibits osteolytic factors induced by osteoblast in co-culture system with breast cancer." dans: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1097-104, (2014) (PubMed).

Xu, Wang, Lu, Xu: "Osteoprotegerin and RANKL in the pathogenesis of rheumatoid arthritis-induced osteoporosis." dans: Rheumatology international, Vol. 32, Issue 11, pp. 3397-403, (2012) (PubMed).

Chen, Li, Liu, Wang, Xie, Liu, Hou, Chang, Du: "Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation." dans: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 19, Issue 11, pp. 1029-34, (2012) (PubMed).

Tang, Zhang, Tang, Qi, Jiang: "Hypoxia induces RANK and RANKL expression by activating HIF-1? in breast cancer cells." dans: Biochemical and biophysical research communications, Vol. 408, Issue 3, pp. 411-6, (2011) (PubMed).

Tang, Sun, Li, Zhou, Yin, Zhou: "Porphyromonas endodontalis lipopolysaccharides induce RANKL by mouse osteoblast in a way different from that of Escherichia coli lipopolysaccharide." dans: Journal of endodontics, Vol. 37, Issue 12, pp. 1653-8, (2011) (PubMed).

Détail du antigène

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Autre désignation: TNFSF11 (TNFSF11 Produits)

Synonymes: CD254 Kit ELISA, ODF Kit ELISA, OPGL Kit ELISA, OPTB2 Kit ELISA, RANKL Kit ELISA, TRANCE Kit ELISA, hRANKL2 Kit ELISA, sOdf Kit ELISA, TNFSF11 Kit ELISA, Ly109l Kit ELISA, OPG Kit ELISA, Trance Kit ELISA, cd254 Kit ELISA, odf Kit ELISA, opgl Kit ELISA, optb2 Kit ELISA, rankl Kit ELISA, sodf Kit ELISA, tnfsf11 Kit ELISA, trance Kit ELISA, TNF superfamily member 11 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 11 Kit ELISA, TNF receptor superfamily member 11b Kit ELISA, TNF superfamily member 11 L homeolog Kit ELISA, TNFSF11 Kit ELISA, Tnfsf11 Kit ELISA, TNFRSF11B Kit ELISA, tnfsf11.L Kit ELISA

Sujet:

Protein Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Background: Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Synonyms: Tumor necrosis factor ligand superfamily member 11,Osteoclast differentiation factor,ODF,Osteoprotegerin ligand,OPGL,Receptor activator of nuclear factor kappa-B ligand,RANKL,TNF-related activation-induced cytokine,TRANCE,CD254,Tumor necrosis factor ligand superfamily member 11, membrane form,Tumor necrosis factor ligand superfamily member 11, soluble form,Tnfsf11,Opgl, Rankl, Trance,

Full Gene Name: Tumor necrosis factor ligand superfamily member 11

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type II membrane protein.

ID gène: 21943

UniProt: O35235

Pathways: Signalisation NF-kappaB