CD137 Kit ELISA

N° du produit ABIN1672885

Détail du produit CD137 Kit ELISA

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Épitope: AA 24-186

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF9/4-1BB

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: L24-Q186

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: L24-Q186

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 4 TNFR-Cys repeats.

Tissue Specificity: Expressed on the surface of activated T-cells.

Plaque: Pre-coated

Protocole: human TNFRSF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF9 has been precoated onto 96-well plates. Standards(NSO, L24-Q186) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF9 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF9 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 241, Standard deviation: 8.9, CV(%): 3.7
• Sample 2: n=16, Mean(pg/ml): 668, Standard deviation: 34, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 1134, Standard deviation: 48.8, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 224, Standard deviation: 11, CV(%): 4.9
• Sample 2: n=24, Mean(pg/ml): 735, Standard deviation: 45.57, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 1268, Standard deviation: 69.8, CV(%): 5.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Autre désignation: TNFRSF9 (TNFRSF9 Produits)

Synonymes: TNFRSF9 Kit ELISA, zgc:136557 Kit ELISA, 4-1BB Kit ELISA, CD137 Kit ELISA, CDw137 Kit ELISA, ILA Kit ELISA, A930040I11Rik Kit ELISA, AA408498 Kit ELISA, AI325004 Kit ELISA, Cd137 Kit ELISA, Ly63 Kit ELISA, tumor necrosis factor receptor superfamily member 9 Kit ELISA, TNF receptor superfamily member 9 Kit ELISA, tumor necrosis factor receptor superfamily, member 9a Kit ELISA, tumor necrosis factor receptor superfamily, member 9 Kit ELISA, TNFRSF9 Kit ELISA, tnfrsf9a Kit ELISA, Tnfrsf9 Kit ELISA

Sujet:

Protein Function: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Background: CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Synonyms: Tumor necrosis factor receptor superfamily member 9,4-1BB ligand receptor,CDw137,T-cell antigen 4-1BB homolog,T-cell antigen ILA,CD137,TNFRSF9,CD137, ILA,

Full Gene Name: Tumor necrosis factor receptor superfamily member 9

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 3604

UniProt: Q07011

Pathways: Cancer Immune Checkpoints