SensoLyte® 390 Cathepsin D Assay Kit

N° du produit ABIN1882438

Détail du produit

Antigène: Cathepsin D (CTSD)

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Components A and B from light and moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

Référence

Poliakov, Strunnikova, Jiang, Martinez, Parikh, Lakkaraju, Thomas, Brooks, Redmond: "Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells." dans: Molecular vision, Vol. 20, pp. 285-300, (2014) (PubMed).

Détail du antigène

Antigène: Cathepsin D (CTSD)

Autre désignation: Cathepsin D (CTSD Produits)

Synonymes: catD Kit, fb93e11 Kit, fj17b09 Kit, wu:fb93e11 Kit, wu:fj17b09 Kit, LOC398557 Kit, Cathd Kit, CatD Kit, LOC100136761 Kit, CTSD Kit, DKFZp469J0315 Kit, DDBDRAFT_0205785 Kit, DDBDRAFT_0215012 Kit, DDB_0205785 Kit, DDB_0215012 Kit, CLN10 Kit, CPSD Kit, CD Kit, cathepsin D Kit, cathepsin D S homeolog Kit, lysosomal aspartic protease Kit, cathepsin D-like Kit, ctsd Kit, LOC398557 Kit, ctsd.S Kit, CatD Kit, LOC5567565 Kit, LOC100136761 Kit, CTSD Kit, Cathd Kit, ctsD Kit, catd Kit, Ctsd Kit, LOC443060 Kit

Sujet: Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.