SensoLyte® 390 Renin Assay Kit

N° du produit ABIN1882549

Détail du produit

Antigène: Renin (REN)

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 390 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by renin, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay is about four fold more sensitive than an EDANS/DABCYL-based assay and can detect 8 ng/mL of renin.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Aliquots are needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Référence

Maeda, Inoguchi, Takei, Sawada, Sasaki, Fujii, Kobayashi, Urata, Nishiyama, Takayanagi: "Inhibition of chymase protects against diabetes-induced oxidative stress and renal dysfunction in hamsters." dans: American journal of physiology. Renal physiology, Vol. 299, Issue 6, pp. F1328-38, (2010) (PubMed).

Détail du antigène

Antigène: Renin (REN)

Autre désignation: Renin (REN Produits)

Synonymes: HNFJ2 Kit, RATRENAA Kit, RENAA Kit, Ren1 Kit, D19352 Kit, Ren Kit, Ren-1 Kit, Ren-A Kit, Ren1c Kit, Ren1d Kit, Rn-1 Kit, Rnr Kit, renin Kit, renin 1 structural Kit, REN Kit, Ren Kit, Ren1 Kit, ren Kit

Sujet: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.