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TNFRSF8 Kit ELISA

TNFRSF8 Reactivité: Souris AA 19-281 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN1889304
  • Antigène Voir toutes TNFRSF8 Kits ELISA
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    Épitope
    AA 19-281
    Reactivité
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD30
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: F19-T281
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: F19-T281
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Detected in thymus and in activated splenocytes.

    Plaque
    Pre-coated
    Protocole
    mouse CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-T281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CD30 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD30 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 90, Standard deviation: 4.77, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 345, Standard deviation: 21.39, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 653, Standard deviation: 46.36, CV(%): 7.1,
    • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 6.63, CV(%): 6.5
    • Sample 2: n=24, Mean(pg/ml): 367, Standard deviation: 27.53, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 721, Standard deviation: 60.56, CV(%): 8.4
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes TNFRSF8 Kits ELISA
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    Autre désignation
    TNFRSF8 (TNFRSF8 Produits)
    Synonymes
    CD30 Kit ELISA, D1S166E Kit ELISA, Ki-1 Kit ELISA, Cd30 Kit ELISA, Ki Kit ELISA, TNFRSF8 Kit ELISA, TNF receptor superfamily member 8 Kit ELISA, tumor necrosis factor receptor superfamily, member 8 Kit ELISA, TNFRSF8 Kit ELISA, Tnfrsf8 Kit ELISA
    Sujet

    Protein Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B (By similarity). .

    Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

    Synonyms: Tumor necrosis factor receptor superfamily member 8,CD30L receptor,Lymphocyte activation antigen CD30,CD30,Tnfrsf8,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 8

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    21941
    UniProt
    Q60846
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