HVEM Kit ELISA

N° du produit ABIN1889391

Détail du produit HVEM Kit ELISA

Antigène: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Épitope: AA 29-207

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF14/HVEM

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: Q29-V207

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: Q29-V207

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse TNFRSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF14 has been precoated onto 96-well plates. Standards(CHO, Q29-V207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF14 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFRSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF14 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 58, Standard deviation: 2.5, CV(%): 4.3
• Sample 2: n=16, Mean(pg/ml): 260, Standard deviation: 9.36, CV(%): 3.6
• Sample 3: n=16, Mean(pg/ml): 594, Standard deviation: 30.3, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 64, Standard deviation: 3.58, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 286, Standard deviation: 12.87, CV(%): 4.5
• Sample 3: n=24, Mean(pg/ml): 654, Standard deviation: 39.24, CV(%): 6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Autre désignation: TNFRSF14 (TNFRSF14 Produits)

Synonymes: ATAR Kit ELISA, CD270 Kit ELISA, HVEA Kit ELISA, HVEM Kit ELISA, LIGHTR Kit ELISA, TR2 Kit ELISA, Atar Kit ELISA, HveA Kit ELISA, Hvem Kit ELISA, Tnfrs14 Kit ELISA, TNF receptor superfamily member 14 Kit ELISA, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Kit ELISA, Tnfrsf14 Kit ELISA, TNFRSF14 Kit ELISA

Classe de substances: Viral Protein

Sujet:

Background: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

Synonyms: Protein Tnfrsf14 ,Tumor necrosis factor receptor superfamily member 14 ,Tnfrsf14 ,

Full Gene Name: tumor necrosis factor receptor superfamily, member 14

ID gène: 230979

UniProt: Q80WM9

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints