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FCN3 Kit ELISA

FCN3 Reactivité: Humain AA 24-299 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
N° du produit ABIN1889410
  • Antigène Voir toutes FCN3 Kits ELISA
    FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
    Épitope
    AA 24-299
    Reactivité
    • 5
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.78-50 ng/mL
    Seuil minimal de détection
    0.78 ng/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Q24-R299
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <20pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Q24-R299
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the ficolin lectin family.

    Tissue Specificity: Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells. .

    Plaque
    Pre-coated
    Protocole
    human Ficolin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been precoated onto 96-well plates. Standards(NSO, Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-3 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-3 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 4.88, Standard deviation: 0.215, CV(%): 4.4
    • Sample 2: n=16, Mean(ng/ml): 12.6, Standard deviation: 0.466, CV(%): 3.7
    • Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.17, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 5.14, Standard deviation: 0.35, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.02, CV(%): 7.5
    • Sample 3: n=24, Mean(ng/ml): 25.7, Standard deviation: 1.98, CV(%): 7.7
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes FCN3 Kits ELISA
    FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
    Autre désignation
    FCN3 (FCN3 Produits)
    Synonymes
    FCNH Kit ELISA, HAKA1 Kit ELISA, ficolin 3 Kit ELISA, ficolin-3 Kit ELISA, FCN3 Kit ELISA, Fcn3 Kit ELISA, fcn3-A Kit ELISA
    Sujet

    Protein Function: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. .

    Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

    Synonyms: Ficolin-3,Collagen/fibrinogen domain-containing lectin 3 p35,Collagen/fibrinogen domain-containing protein 3,Hakata antigen,FCN3,FCNH, HAKA1,

    Full Gene Name: Ficolin-3

    Cellular Localisation: Secreted. Found in blood plasma, bronchus, alveolus and bile duct.
    ID gène
    8547
    UniProt
    O75636
    Pathways
    Système du Complément
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