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FCGR2B Kit ELISA

FCGR2B Reactivité: Humain AA 46-217 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889450
  • Antigène Voir toutes FCGR2B Kits ELISA
    FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
    Épitope
    AA 46-217
    Reactivité
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    46.9-3000 pg/mL
    Seuil minimal de détection
    46.9 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD32/FCGR2b/c
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: A46-P217
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: A46-P217
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    Tissue Specificity: Isoform IIC1 is detected in monocytes, macrophages, polymorphonuclear cells and natural killer cells.

    Plaque
    Pre-coated
    Protocole
    human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 147, Standard deviation: 6.62, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 890, Standard deviation: 32.93, CV(%): 3.7
    • Sample 3: n=16, Mean(pg/ml): 1865, Standard deviation: 80.2, CV(%): 4.3,
    • Sample 1: n=24, Mean(pg/ml): 168, Standard deviation: 9.75, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 904, Standard deviation: 41.6, CV(%): 4.6
    • Sample 3: n=24, Mean(pg/ml): 2162, Standard deviation: 112.42, CV(%): 5.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes FCGR2B Kits ELISA
    FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
    Autre désignation
    FCGR2B (FCGR2B Produits)
    Synonymes
    CD32 Kit ELISA, CD32B Kit ELISA, FCG2 Kit ELISA, FCGR2 Kit ELISA, IGFR2 Kit ELISA, FCGR2B Kit ELISA, AI528646 Kit ELISA, F630109E10Rik Kit ELISA, Fc[g]RII Kit ELISA, FcgRII Kit ELISA, Fcgr2 Kit ELISA, Fcgr2a Kit ELISA, Fcr-2 Kit ELISA, Fcr-3 Kit ELISA, Ly-17 Kit ELISA, Ly-m20 Kit ELISA, LyM-1 Kit ELISA, Lym-1 Kit ELISA, FCRII Kit ELISA, FcRII Kit ELISA, Fc fragment of IgG receptor IIb Kit ELISA, Fc fragment of IgG, low affinity IIb, receptor (CD32) Kit ELISA, Fc receptor, IgG, low affinity IIb Kit ELISA, low affinity immunoglobulin gamma Fc region receptor II Kit ELISA, FCGR2B Kit ELISA, Fcgr2b Kit ELISA, LOC100856270 Kit ELISA
    Sujet

    Protein Function: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells.

    Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

    Synonyms: Low affinity immunoglobulin gamma Fc region receptor II-c,IgG Fc receptor II-c,CDw32,Fc-gamma RII-c,Fc-gamma-RIIc,FcRII-c,CD32,FCGR2C,CD32, FCG2, IGFR2,

    Full Gene Name: Low affinity immunoglobulin gamma Fc region receptor II-c

    Cellular Localisation: Isoform IIC4: Cytoplasm.
    ID gène
    2213, 9103
    UniProt
    P31995
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling
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