SERPINE1 Kit ELISA (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1)

Details for Product SERPINE1 ELISA Kit No. ABIN2010733, Fournisseur: Connectez-vous pour afficher
Antigène
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • SERPINE1
  • si:ch211-138a11.1
  • Planh1
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin family E member 1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • SERPINE1
  • serpine1.L
  • serpine1
  • Serpine1
Reactivité
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
42
23
22
13
11
7
5
5
4
3
2
2
2
1
Type de méthode
Sandwich ELISA
Gamme de detection
0.05-50 ng/mL
Seuil minimal de détection
0.05 ng/mL
Application
ELISA
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Fonction The sensitive quantitative measurement of functionally active rat PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit.
Analytical Method Quantitative
Méthode de détection Colorimetric
Ingrédients All reagents and standards are provided in this kit.
Plasmids, Primers & others Plasmids, Primers & others SERPINE1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation PAI-1 (SERPINE1 ELISA Kit Extrait)
Sujet Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
ID gène 24617
UniProt P20961
Pathways Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
Plaque Pre-coated
Protocole The concentration level of PAI-1 activity in rat plasma was found to be 1.0 ng/mL. The assay measures active PAI-1 in the 0.05-50 ng/mL range. Samples giving rat PAI-1 levels above 50 ng/mL should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. Cross-reactivity: 12percent porcine PAI-1, 100percent mouse PAI-1. After appropriate washing steps, anti-rat PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples.
Restrictions For Research Use only
Stock 4 °C
Produit citée dans: Rancourt, Veress, Ahmad, Hendry-Hofer, Rioux, Garlick, White: "Tissue factor pathway inhibitor prevents airway obstruction, respiratory failure and death due to sulfur mustard analog inhalation." dans: Toxicology and applied pharmacology, Vol. 272, Issue 1, pp. 86-95, 2013 (PubMed).

Lavrentiadou, Tsantarliotou, Zervos, Nikolaidis, Georgiadis, Taitzoglou: "CCl4 induces tissue-type plasminogen activator in rat brain; protective effects of oregano, rosemary or vitamin E." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 61, pp. 196-202, 2013 (PubMed).

Lu, Roth, Malle, Ganey: "Roles of the hemostatic system and neutrophils in liver injury from co-exposure to amiodarone and lipopolysaccharide." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 136, Issue 1, pp. 51-62, 2013 (PubMed).

Zou, Devi, Sparkenbaugh, Younis, Roth, Ganey: "Hepatotoxic interaction of sulindac with lipopolysaccharide: role of the hemostatic system." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 108, Issue 1, pp. 184-93, 2009 (PubMed).

Huang, Border, Lawrence, Noble: "Mechanisms underlying the antifibrotic properties of noninhibitory PAI-1 (PAI-1R) in experimental nephritis." dans: American journal of physiology. Renal physiology, Vol. 297, Issue 4, pp. F1045-54, 2009 (PubMed).

Deng, Lu, Lehman-McKeeman, Malle, Crandall, Ganey, Roth: "p38 mitogen-activated protein kinase-dependent tumor necrosis factor-alpha-converting enzyme is important for liver injury in hepatotoxic interaction between lipopolysaccharide and ranitidine." dans: The Journal of pharmacology and experimental therapeutics, Vol. 326, Issue 1, pp. 144-52, 2008 (PubMed).

Deng, Luyendyk, Zou, Lu, Malle, Ganey, Roth: "Neutrophil interaction with the hemostatic system contributes to liver injury in rats cotreated with lipopolysaccharide and ranitidine." dans: The Journal of pharmacology and experimental therapeutics, Vol. 322, Issue 2, pp. 852-61, 2007 (PubMed).

Bergheim, Luyendyk, Steele, Russell, Guo, Roth, Arteel: "Metformin prevents endotoxin-induced liver injury after partial hepatectomy." dans: The Journal of pharmacology and experimental therapeutics, Vol. 316, Issue 3, pp. 1053-61, 2006 (PubMed).

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