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FNDC5 Kit ELISA

FNDC5 Reactivité: Humain Colorimetric 3.12-200 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN2039309
  • Antigène Voir toutes FNDC5 Kits ELISA
    FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
    Reactivité
    • 10
    • 9
    • 9
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Gamme de detection
    3.12-200 ng/mL
    Seuil minimal de détection
    3.12 ng/mL
    Application
    ELISA
    Type d'échantillon
    Serum, Plasma, Tissue Homogenate
    Analytical Method
    Quantitative
    Réactivité croisée (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensibilité
    0.78 ng/mL
    Ingrédients
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Indications d'application
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Commentaires

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Conseil sur la manipulation
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Date de péremption
    6 months
  • Li, Xu, Wang, Liao, Li, Yang, Gao: "Free androgen index and Irisin in polycystic ovary syndrome." dans: Journal of endocrinological investigation, Vol. 39, Issue 5, pp. 549-56, (2017) (PubMed).

    Perakakis, Triantafyllou, Fernández-Real, Huh, Park, Seufert, Mantzoros: "Physiology and role of irisin in glucose homeostasis." dans: Nature reviews. Endocrinology, Vol. 13, Issue 6, pp. 324-337, (2017) (PubMed).

    Dinas, Lahart, Timmons, Svensson, Koutedakis, Flouris, Metsios: "Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review." dans: F1000Research, Vol. 6, pp. 286, (2017) (PubMed).

    Li, Yang, Zhou, Fang, Hu, Zhu, Wang, Liu, Li, Liu, Yang, Li: "Elevated circulating levels of irisin and the effect of metformin treatment in women with polycystic ovary syndrome." dans: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 4, pp. 1485-93, (2015) (PubMed).

    Palacios-González, Vadillo-Ortega, Polo-Oteyza, Sánchez, Ancira-Moreno, Romero-Hidalgo, Meráz, Antuna-Puente: "Irisin levels before and after physical activity among school-age children with different BMI: a direct relation with leptin." dans: Obesity (Silver Spring, Md.), Vol. 23, Issue 4, pp. 729-32, (2015) (PubMed).

    Aydin, Aydin, Kobat, Kalayci, Eren, Yilmaz, Kuloglu, Gul, Secen, Alatas, Baydas: "Decreased saliva/serum irisin concentrations in the acute myocardial infarction promising for being a new candidate biomarker for diagnosis of this pathology." dans: Peptides, Vol. 56, pp. 141-5, (2015) (PubMed).

  • Antigène Voir toutes FNDC5 Kits ELISA
    FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
    Autre désignation
    Irisin (FNDC5 Produits)
    Synonymes
    FRCP2 Kit ELISA, irisin Kit ELISA, 1500001L03Rik Kit ELISA, AI836596 Kit ELISA, C87088 Kit ELISA, PeP Kit ELISA, Pxp Kit ELISA, fndc5 Kit ELISA, si:dkey-264p5.1 Kit ELISA, zgc:158684 Kit ELISA, fibronectin type III domain containing 5 Kit ELISA, fibronectin type III domain containing 5b Kit ELISA, FNDC5 Kit ELISA, Fndc5 Kit ELISA, fndc5b Kit ELISA
    Pathways
    Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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