HIF1A Kit ELISA

N° du produit ABIN2043725

Détail du produit HIF1A Kit ELISA

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivité: Lapin

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.063 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Hypoxia Inducible Factor 1 Alpha (HIF1a) BioAssay-ELISA Kit (Rabbit) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: Hypoxia Inducible Factor 1 alpha (HIF1a) (HIF1A Produits)

Synonymes: hif1a Kit ELISA, AA959795 Kit ELISA, HIF1alpha Kit ELISA, MOP1 Kit ELISA, bHLHe78 Kit ELISA, HIF-1A Kit ELISA, HIF-1alpha Kit ELISA, HIF1 Kit ELISA, HIF1-ALPHA Kit ELISA, PASD8 Kit ELISA, HIF1-alpha Kit ELISA, hif-1a Kit ELISA, hypoxia inducible factor 1 alpha Kit ELISA, hypoxia inducible factor 1, alpha subunit Kit ELISA, hypoxia inducible factor 1 alpha subunit Kit ELISA, HIF (Hypoxia Inducible Factor) homologa Kit ELISA, hif-1a Kit ELISA, Hif1a Kit ELISA, HIF1A Kit ELISA, hif1a Kit ELISA, hif-1 Kit ELISA

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg