MGMT Kit ELISA

N° du produit ABIN2044129

Détail du produit MGMT Kit ELISA

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 27 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: O-6-Methylguanine DNA Methyltransferase (MGMT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: O-6-Methylguanine DNA Methyltransferase (MGMT) (MGMT Produits)

Synonymes: AGT Kit ELISA, AI267024 Kit ELISA, Agat Kit ELISA, O-6-methylguanine-DNA methyltransferase Kit ELISA, MGMT Kit ELISA, Mgmt Kit ELISA

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus