CytoSelect™ 96-well Cell Migration and Invasion Assay (8 μm), Fluorometric, Combo Kit

N° du produit ABIN2344856

Détail du produit

Reactivité: Mammifères

Méthode de détection: Fluorometric

Application: Cellular Assay (CA)

Marque: CytoSelect™

Type d'échantillon: Serum, Cell Samples

Analytical Method: Quantitative

Attributs du produit: CytoSelect™ 96-well Cell Migration and Invasion Assay Kit utilizes a polycarbonate membrane plate (8 μm pore size) or basement membrane-coated inserts to assay the migratory or invasive properties of cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-migratory or non-invasive cells (i.e. cotton swabbing). Any migratory or invasive cells are first dissociated from the membrane, then lysed and detected with CyQuant® GR Dye. The CytoSelect™ 96-well Cell Migration and Invasion Assay Kit provides a robust system for the quantitative determination of cell migration. Each assay contains sufficient reagents for the evaluation of 96 samples. The 8 μm pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 μm) is recommended.

Ingrédients:

1. 96-well Cell Migration Plate : One sterile 96-well plate (see Figure 1 for components)
2. 96-well Cell Invasion Plate : One sterile 96-well plate containing ECM-coated inserts (see Figure 1 for components)
3. 96-well Cell Harvesting Tray : Two 96-well trays
4. Cell Detachment Solution : Two 20 mL bottles
5. 4X Lysis Buffer : Two 10 mL bottles
6. CyQuant® GR Dye : Two 75 μL tubes Top Plate Cover Middle Migration Plate Membrane Chamber Bottom Feeder Tray Figure 1: Components of the 96-well Cell Migration Plate.

Matériel non inclus:

1. Migratory cell lines
2. Cell culture medium 3
3. Serum free medium, such as DMEM containing 0.5 % BSA, 2 mM CaCl2 and 2 mM MgCl2
4. FBS or desired chemoattractant
5. Cell culture incubator (37 °C, 5 % CO2 atmosphere)
6. Light microscope
7. 96-well plate suitable for a fluorescence plate reader
8. Fluorescence plate reader

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

• Fully quantify chemotaxis and cell invasion with no manual cell counting
• Includes two plates with 8 μm membrane inserts: one uncoated for chemotaxis and one precoated on top of the membrane with ECM matrix (basement membrane) for cell invasion

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store all components at 4°C.

Référence

Zecchini, Madhu, Russell, Pértega-Gomes, Warren, Gaude, Borlido, Stark, Ireland-Zecchini, Rao, Scott, Boren, Massie, Asim, Brindle, Griffiths, Frezza, Neal, Mills: "Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer." dans: The EMBO journal, Vol. 33, Issue 12, pp. 1365-82, (2014) (PubMed).

Sharma, Massie, Butter, Mann, Bon, Ramos-Montoya, Menon, Stark, Lamb, Scott, Warren, Neal, Mills: "The ETS family member GABP? modulates androgen receptor signalling and mediates an aggressive phenotype in prostate cancer." dans: Nucleic acids research, Vol. 42, Issue 10, pp. 6256-69, (2014) (PubMed).

Ardiani, Gameiro, Palena, Hamilton, Kwilas, King, Schlom, Hodge: "Vaccine-mediated immunotherapy directed against a transcription factor driving the metastatic process." dans: Cancer research, Vol. 74, Issue 7, pp. 1945-57, (2014) (PubMed).

Axlund, Lambert, Nordeen: "HOXC8 inhibits androgen receptor signaling in human prostate cancer cells by inhibiting SRC-3 recruitment to direct androgen target genes." dans: Molecular cancer research : MCR, Vol. 8, Issue 12, pp. 1643-55, (2010) (PubMed).

Alfano, Leppla, Liu, Bugge, Meininger, Lairmore, Mulne, Davis, Duesbery, Frankel: "Matrix metalloproteinase-activated anthrax lethal toxin inhibits endothelial invasion and neovasculature formation during in vitro morphogenesis." dans: Molecular cancer research : MCR, Vol. 7, Issue 4, pp. 452-61, (2009) (PubMed).

Eckstein, Servan, Girard, Cai, von Jonquieres, Jaehde, Kassack, Gazdar, Minna, Royer: "Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells." dans: The Journal of biological chemistry, Vol. 283, Issue 2, pp. 739-50, (2008) (PubMed).

Détail du antigène

Sujet: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant, these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased.