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IL12B Kit ELISA

IL12B Reactivité: Rat Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN2642322
  • Antigène Voir toutes IL12B Kits ELISA
    IL12B (Interleukin 12 beta (IL12B))
    Reactivité
    • 8
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25-2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Fonction
    For the quantitative determination of rat interleukin 12 (IL-12/P40) concentrations in serum, plasma and tissue homogenates.
    Type d'échantillon
    Serum, Plasma, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of rat IL-12/P40.
    Réactivité croisée (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensibilité
    7.81 pg/mL
    Ingrédients
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Indications d'application
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Commentaires

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12/P40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12/P40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12/P40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12/P40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Précision du teste
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Conseil sur la manipulation
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Date de péremption
    6 months
  • Zeng, Qiao, Lv, Fan, Liu, Xie: "High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis." dans: Drug design, development and therapy, Vol. 11, pp. 3097-3104, (2018) (PubMed).

  • Antigène Voir toutes IL12B Kits ELISA
    IL12B (Interleukin 12 beta (IL12B))
    Autre désignation
    Interleukin 12,IL-12/P40 (IL12B Produits)
    Synonymes
    CLMF Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12 Kit ELISA, IL-12 p40 Kit ELISA, il12b Kit ELISA, il12p40.b Kit ELISA, zgc:152789 Kit ELISA, IL-12.p40 Kit ELISA, IL12p40 Kit ELISA, IL12B Kit ELISA, CLMF p40 Kit ELISA, interleukin 12B Kit ELISA, interleukin 12b Kit ELISA, interleukin 12Ba Kit ELISA, IL12B Kit ELISA, Il12b Kit ELISA, il12ba Kit ELISA
    Pathways
    Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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