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CD137 Kit ELISA

TNFRSF9 Reactivité: Souris Colorimetric Sandwich ELISA 6-1.500 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702796
  • Antigène Voir toutes CD137 (TNFRSF9) Kits ELISA
    CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
    Reactivité
    • 6
    • 4
    • 2
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    6-1.500 pg/mL
    Seuil minimal de détection
    6 pg/mL
    Application
    ELISA
    Fonction
    Mouse 4-1BB (TNFRSF9/CD137) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    Sensibilité
    6 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples3-30 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CD137 (TNFRSF9) Kits ELISA
    CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
    Autre désignation
    4-1BB (TNFRSF9 Produits)
    Synonymes
    TNFRSF9 Kit ELISA, zgc:136557 Kit ELISA, 4-1BB Kit ELISA, CD137 Kit ELISA, CDw137 Kit ELISA, ILA Kit ELISA, A930040I11Rik Kit ELISA, AA408498 Kit ELISA, AI325004 Kit ELISA, Cd137 Kit ELISA, Ly63 Kit ELISA, tumor necrosis factor receptor superfamily member 9 Kit ELISA, TNF receptor superfamily member 9 Kit ELISA, tumor necrosis factor receptor superfamily, member 9a Kit ELISA, tumor necrosis factor receptor superfamily, member 9 Kit ELISA, TNFRSF9 Kit ELISA, tnfrsf9a Kit ELISA, Tnfrsf9 Kit ELISA
    Sujet
    Gene Names: Tnfrsf9 Cd137 Ila Ly63
    Protein names: Tumor necrosis factor receptor superfamily member 9 (4-1BB ligand receptor) (T-cell antigen 4-1BB) (CD antigen CD137)
    ID gène
    21942
    UniProt
    P20334
    Pathways
    Cancer Immune Checkpoints
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