AGT Kit ELISA

N° du produit ABIN2702821

Détail du produit AGT Kit ELISA

Antigène: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.22-300 ng/mL

Seuil minimal de détection: 1.22 ng/mL

Application: ELISA

Fonction: Human Angiotensinogen (SERPINA8) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Angiotensinogen (AGT/SERPINA8)

Sensibilité: 1.22 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples100 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Autre désignation: Angiotensinogen (AGT Produits)

Synonymes: ANHU Kit ELISA, SERPINA8 Kit ELISA, AI265500 Kit ELISA, AngI Kit ELISA, AngII Kit ELISA, Aogen Kit ELISA, Serpina8 Kit ELISA, ANRT Kit ELISA, Ang Kit ELISA, PAT Kit ELISA, wu:fb62f06 Kit ELISA, wu:fj87b02 Kit ELISA, zgc:111892 Kit ELISA, AGT Kit ELISA, angt Kit ELISA, ANGT Kit ELISA, angiotensinogen Kit ELISA, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Kit ELISA, AGT Kit ELISA, Agt Kit ELISA, agt Kit ELISA

Sujet: Gene Names: AGT SERPINA8
Protein names: Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I), Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II), Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II), Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV), Angiotensin 1-9, Angiotensin 1-7, Angiotensin 1-5, Angiotensin 1-4]

ID gène: 183

UniProt: P01019

Pathways: Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity