Apo-B100 Kit ELISA

N° du produit ABIN2702829

Détail du produit Apo-B100 Kit ELISA

Antigène: Apo-B100 (Apoprotein B100 (Apo-B100))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2.5-600 ng/mL

Seuil minimal de détection: 2.5 ng/mL

Application: ELISA

Fonction: Human ApoB100 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human ApoB100. Other species not determined.

Sensibilité: 2.5 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples10,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

For example, add 2 μL of serum or plasma into a tube with 98 μL 1X Assay Diluent to prepare a 50-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 50-fold diluted sample into a tube with 398 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Apo-B100 (Apoprotein B100 (Apo-B100))

Autre désignation: ApoB100 (Apo-B100 Produits)

Synonymes: Ac1-060 Kit ELISA, Apo B-100 Kit ELISA, ApoB-100 Kit ELISA, ApoB-48 Kit ELISA, apolipoprotein B Kit ELISA, Apob Kit ELISA

Sujet: Gene Names: APOB
Protein names: Apolipoprotein B-100 (Apo B-100) [Cleaved into: Apolipoprotein B-48 (Apo B-48)]

ID gène: 338

UniProt: P04114