APOC3 Kit ELISA

N° du produit ABIN2702832

Détail du produit APOC3 Kit ELISA

Antigène: APOC3 (Apolipoprotein C-III (APOC3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2.5-10.000 pg/mL

Seuil minimal de détection: 2.5 pg/mL

Application: ELISA

Fonction: Human ApoC3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF

Sensibilité: 2.5 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples500,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

For example, add 2 μL of serum/plasma into a tube with 998.0 μL Assay Diluent to prepare a 500-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 500-fold diluted sample into a tube with 1998 μL Assay Diluent to prepare a final 500,000 fold diluted sample.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: APOC3 (Apolipoprotein C-III (APOC3))

Autre désignation: ApoC3 (APOC3 Produits)

Synonymes: APOC3 Kit ELISA, APOCIII Kit ELISA, HALP2 Kit ELISA, Apoc Kit ELISA, ApoC-III Kit ELISA, apo-CIII Kit ELISA, Apo-CIII Kit ELISA, apolipoprotein C3 Kit ELISA, apolipoprotein C-III Kit ELISA, APOC3 Kit ELISA, Apoc3 Kit ELISA

Sujet: Gene Names: APOC3
Protein names: Apolipoprotein C-III (Apo-CIII) (ApoC-III) (Apolipoprotein C3)

ID gène: 345

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism