PD-L1 Kit ELISA

N° du produit ABIN2702842

Détail du produit PD-L1 Kit ELISA

Antigène: PD-L1 (CD274 (PD-L1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46-3000 pg/mL

Seuil minimal de détection: 46 pg/mL

Application: ELISA

Fonction: Human B7-H1 (CD274) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human B7-H1 (CD274)

Sensibilité: 46 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: B7H1/PD-L1 (PD-L1 Produits)

Synonymes: B7-H Kit ELISA, B7H1 Kit ELISA, PD-L1 Kit ELISA, PDCD1L1 Kit ELISA, PDCD1LG1 Kit ELISA, PDL1 Kit ELISA, A530045L16Rik Kit ELISA, B7h1 Kit ELISA, Pdcd1l1 Kit ELISA, Pdcd1lg1 Kit ELISA, Pdl1 Kit ELISA, RGD1566211 Kit ELISA, CD274 molecule Kit ELISA, CD274 antigen Kit ELISA, programmed cell death 1 ligand 2 Kit ELISA, CD274 Kit ELISA, Cd274 Kit ELISA, PDCD1LG2 Kit ELISA

Sujet: Gene Names: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1
Protein names: Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)

ID gène: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints