Endothelin 1 Kit ELISA

N° du produit ABIN2702980

Détail du produit Endothelin 1 Kit ELISA

Antigène: Endothelin 1 (EDN1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 10-1000 pg/mL

Seuil minimal de détection: 10 pg/mL

Application: ELISA

Fonction: Human Endothelin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Endothelin-1 (EDN1)

Sensibilité: 10 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour Endothelin 1 Kit ELISA (ABIN2702980)

Pahari, Gu, van Oeveren, El-Essawi, Harringer, Brouwer: "Effect of minimized perfusion circuit on brain injury markers carnosinase and brain-type fatty binding protein in coronary artery bypass grafting patients." dans: Artificial organs, Vol. 37, Issue 2, pp. 128-35, (2013) (PubMed).

Said, Smith, Sanchez-Carbayo, Theodorescu: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." dans: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).

Détail du antigène

Antigène: Endothelin 1 (EDN1)

Autre désignation: Endothelin-1 (EDN1 Produits)

Synonymes: et1 Kit ELISA, et-1 Kit ELISA, Edn1 Kit ELISA, EDN Kit ELISA, ET-1 Kit ELISA, ET1 Kit ELISA, PPET1 Kit ELISA, HDLCQ7 Kit ELISA, preproET Kit ELISA, Et1 Kit ELISA, fb14d01 Kit ELISA, wu:fb14d01 Kit ELISA, PPET-1 Kit ELISA, EDN1 Kit ELISA, edn1 Kit ELISA, edn1-a Kit ELISA, edn1-b Kit ELISA, endothelin 1 Kit ELISA, endothelin 1 S homeolog Kit ELISA, endothelin 1 L homeolog Kit ELISA, edn1 Kit ELISA, EDN1 Kit ELISA, Edn1 Kit ELISA, edn1.S Kit ELISA, edn1.L Kit ELISA

Sujet: Gene Names: EDN1
Protein names: Endothelin-1 (Preproendothelin-1) (PPET1) [Cleaved into: Endothelin-1 (ET-1), Big endothelin-1]

ID gène: 1906

UniProt: P05305

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size