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FGF12 Kit ELISA

FGF12 Reactivité: Humain Colorimetric Sandwich ELISA 32-8.000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703006
  • Antigène Voir toutes FGF12 Kits ELISA
    FGF12 (Fibroblast Growth Factor 12 (FGF12))
    Reactivité
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    32-8.000 pg/mL
    Seuil minimal de détection
    32 pg/mL
    Application
    ELISA
    Fonction
    Human FGF-12 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes human FGF-12. Other species not yet determined.
    Sensibilité
    32 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FGF12 Kits ELISA
    FGF12 (Fibroblast Growth Factor 12 (FGF12))
    Autre désignation
    FGF-12 (FGF12 Produits)
    Synonymes
    FHF1 Kit ELISA, FHF-1 Kit ELISA, MGC84150 Kit ELISA, FGF12 Kit ELISA, fgf12 Kit ELISA, zgc:153104 Kit ELISA, fhf1 Kit ELISA, fgf12b Kit ELISA, DKFZp459P139 Kit ELISA, FGF12B Kit ELISA, AV114868 Kit ELISA, FGF-12 Kit ELISA, Fhf1 Kit ELISA, Fhf1b Kit ELISA, fibroblast growth factor 12 Kit ELISA, fibroblast growth factor 12 S homeolog Kit ELISA, fibroblast growth factor 12a Kit ELISA, FGF12 Kit ELISA, fgf12.S Kit ELISA, fgf12a Kit ELISA, fgf12 Kit ELISA, Fgf12 Kit ELISA
    Sujet
    Gene Names: FGF12 FGF12B FHF1
    Protein names: Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor homologous factor 1) (FHF-1) (Myocyte-activating factor)
    ID gène
    2257
    UniProt
    P61328
    Pathways
    Negative Regulation of Transporter Activity
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