IL1F9 Kit ELISA
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- Antigène Voir toutes IL1F9 Kits ELISA
- IL1F9 (Interleukin 1 Family Member 9 (IL1F9))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 13-3000 pg/mL
- Seuil minimal de détection
- 13 pg/mL
- Application
- ELISA
- Fonction
- Human IL-36 gamma (IL-1 F9) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33)
- Sensibilité
- 13 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes IL1F9 Kits ELISA
- IL1F9 (Interleukin 1 Family Member 9 (IL1F9))
- Autre désignation
- IL-1F9 (IL1F9 Produits)
- Synonymes
- IL-1F9 Kit ELISA, IL-1H1 Kit ELISA, IL-1RP2 Kit ELISA, IL1E Kit ELISA, IL1F9 Kit ELISA, IL1H1 Kit ELISA, IL1RP2 Kit ELISA, Il36g Kit ELISA, Il1f9 Kit ELISA, RGD1563019 Kit ELISA, IL36G Kit ELISA, interleukin 36 gamma Kit ELISA, interleukin 1 family, member 9 Kit ELISA, interleukin 36, gamma Kit ELISA, interleukin-36 gamma Kit ELISA, IL36G Kit ELISA, Il1f9 Kit ELISA, Il36g Kit ELISA, LOC100065031 Kit ELISA
- Sujet
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Gene Names: IL36G IL1E IL1F9 IL1H1 IL1RP2 UNQ2456/PRO5737
Protein names: Interleukin-36 gamma (IL-1-related protein 2) (IL-1RP2) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 9) (IL-1F9) (Interleukin-1 homolog 1) (IL-1H1)
- ID gène
- 56300
- UniProt
- Q9NZH8
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