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Periostin Kit ELISA

POSTN Reactivité: Humain Colorimetric Sandwich ELISA 80 pg/mL-60 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703410
  • Antigène Voir toutes Periostin (POSTN) Kits ELISA
    Periostin (POSTN)
    Reactivité
    • 8
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    80 pg/mL-60 ng/mL
    Seuil minimal de détection
    80 pg/mL
    Application
    ELISA
    Fonction
    Human Periostin ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects human Periostin. Other species not determined.
    Sensibilité
    80 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2-20 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Zhang, Zhang, Sun, Dong, Xu, Dai: "Periostin: A Downstream Mediator of EphB4-Induced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells." dans: Stem cells international, Vol. 2016, pp. 7241829, (2016) (PubMed).

  • Antigène Voir toutes Periostin (POSTN) Kits ELISA
    Periostin (POSTN)
    Autre désignation
    Periostin (POSTN Produits)
    Synonymes
    fa99h07 Kit ELISA, pn Kit ELISA, postn Kit ELISA, wu:fa99h07 Kit ELISA, wu:fc70f09 Kit ELISA, zgc:153873 Kit ELISA, LOC100008945 Kit ELISA, osf-2 Kit ELISA, pdlpostn Kit ELISA, periostin Kit ELISA, OSF-2 Kit ELISA, OSF2 Kit ELISA, PDLPOSTN Kit ELISA, PN Kit ELISA, RP11-412K4.1 Kit ELISA, A630052E07Rik Kit ELISA, AI747096 Kit ELISA, Osf2 Kit ELISA, PLF Kit ELISA, peri Kit ELISA, Plf Kit ELISA, periostin Kit ELISA, periostin, osteoblast specific factor b Kit ELISA, periostin, osteoblast specific factor Kit ELISA, POSTN Kit ELISA, postnb Kit ELISA, postn Kit ELISA, Postn Kit ELISA
    Sujet
    Gene Names: POSTN OSF2
    Protein names: Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
    ID gène
    10631
    UniProt
    Q15063
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