SERPING1 Kit ELISA

N° du produit ABIN2703455

Détail du produit SERPING1 Kit ELISA

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2.4-600 ng/mL

Seuil minimal de détection: 2.4 ng/mL

Application: ELISA

Fonction: Human SERPING1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes human SERPING1. Other species not yet determined.

Sensibilité: 2.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples600 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))

Autre désignation: Serpin G1 (SERPING1 Produits)

Synonymes: fb64f02 Kit ELISA, im:7159527 Kit ELISA, si:dkeyp-110g5.2 Kit ELISA, wu:fb60c09 Kit ELISA, wu:fb62a08 Kit ELISA, wu:fb64f02 Kit ELISA, LOC100136072 Kit ELISA, C1-INH Kit ELISA, C1INH Kit ELISA, C1nh Kit ELISA, C1IN Kit ELISA, C1NH Kit ELISA, HAE1 Kit ELISA, HAE2 Kit ELISA, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 Kit ELISA, C1 inhibitor Kit ELISA, serpin family G member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade G, member 1 Kit ELISA, serping1 Kit ELISA, LOC100136072 Kit ELISA, SERPING1 Kit ELISA, Serping1 Kit ELISA

Sujet: Gene Names: SERPING1 C1IN C1NH
Protein names: Plasma protease C1 inhibitor (C1 Inh) (C1Inh) (C1 esterase inhibitor) (C1-inhibiting factor) (Serpin G1)

ID gène: 710

UniProt: P05155

Pathways: Système du Complément