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TIM3 Kit ELISA
TIM 3
Reactivité: Humain
Colorimetric
Sandwich ELISA
10-2500 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703498
Détail du produit TIM3 Kit ELISA
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Antigène
Voir toutes TIM3 (TIM 3) Kits ELISA
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Reactivité
Toutes les réactivités sur TIM3 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
10-2500 pg/mL
Seuil minimal de détection
10 pg/mL
Application
ELISA
Fonction
Human TIM-3 (KIM-3) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Human T Cell Immunoglobulin Mucin-3 (HAVCR2 / KIM-3)
Sensibilité
10 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples5 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TIM3 (TIM 3) Kits ELISA
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Autre désignation
TIM-3 (TIM 3 Produits )
Synonymes
HAVCR2 Kit ELISA, MGC140131 Kit ELISA, HAVcr-2 Kit ELISA, KIM-3 Kit ELISA, TIM3 Kit ELISA, TIMD-3 Kit ELISA, TIMD3 Kit ELISA, Tim-3 Kit ELISA, TIM-3 Kit ELISA, Tim3 Kit ELISA, Timd3 Kit ELISA, tim3 Kit ELISA, hepatitis A virus cellular receptor 2 Kit ELISA, HAVCR2 Kit ELISA, Havcr2 Kit ELISA
Classe de substances
Virus
Sujet
Gene Names: HAVCR2 TIM3 TIMD3 Protein names: Hepatitis A virus cellular receptor 2 (HAVcr-2) (T-cell immunoglobulin and mucin domain-containing protein 3) (TIMD-3) (T-cell immunoglobulin mucin receptor 3) (TIM-3) (T-cell membrane protein 3)
ID gène
84868
UniProt
Q8TDQ0
Pathways
Regulation of Lipid Metabolism by PPARalpha , Cancer Immune Checkpoints
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