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TIM3 Kit ELISA

TIM 3 Reactivité: Humain Colorimetric Sandwich ELISA 10-2500 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703498
  • Antigène Voir toutes TIM3 (TIM 3) Kits ELISA
    TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
    Reactivité
    • 3
    • 3
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    10-2500 pg/mL
    Seuil minimal de détection
    10 pg/mL
    Application
    ELISA
    Fonction
    Human TIM-3 (KIM-3) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human T Cell Immunoglobulin Mucin-3 (HAVCR2 / KIM-3)
    Sensibilité
    10 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples5 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes TIM3 (TIM 3) Kits ELISA
    TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
    Autre désignation
    TIM-3 (TIM 3 Produits)
    Synonymes
    HAVCR2 Kit ELISA, MGC140131 Kit ELISA, HAVcr-2 Kit ELISA, KIM-3 Kit ELISA, TIM3 Kit ELISA, TIMD-3 Kit ELISA, TIMD3 Kit ELISA, Tim-3 Kit ELISA, TIM-3 Kit ELISA, Tim3 Kit ELISA, Timd3 Kit ELISA, tim3 Kit ELISA, hepatitis A virus cellular receptor 2 Kit ELISA, HAVCR2 Kit ELISA, Havcr2 Kit ELISA
    Classe de substances
    Virus
    Sujet
    Gene Names: HAVCR2 TIM3 TIMD3
    Protein names: Hepatitis A virus cellular receptor 2 (HAVcr-2) (T-cell immunoglobulin and mucin domain-containing protein 3) (TIMD-3) (T-cell immunoglobulin mucin receptor 3) (TIM-3) (T-cell membrane protein 3)
    ID gène
    84868
    UniProt
    Q8TDQ0
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints
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