TNFAIP6 Kit ELISA

N° du produit ABIN2703540

Détail du produit TNFAIP6 Kit ELISA

Antigène: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.2-50 ng/mL

Seuil minimal de détection: 0.2 ng/mL

Application: ELISA

Fonction: Human TSG-6 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes human TSG-6. Other species not yet determined.

Sensibilité: 0.2 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour TNFAIP6 Kit ELISA (ABIN2703540)

Um, Kim, Lee, Song, Seo: "TSG-6 secreted by mesenchymal stem cells suppresses immune reactions influenced by BMP-2 through p38 and MEK mitogen-activated protein kinase pathway." dans: Cell and tissue research, Vol. 368, Issue 3, pp. 551-561, (2018) (PubMed).

Kui, Chan, Lee: "TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells." dans: Mediators of inflammation, Vol. 2017, pp. 7462945, (2017) (PubMed).

Détail du antigène

Antigène: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Autre désignation: TSG-6 (TNFAIP6 Produits)

Synonymes: Tnfip6 Kit ELISA, TSG-6 Kit ELISA, Tsg6 Kit ELISA, TSG6 Kit ELISA, TNF alpha induced protein 6 Kit ELISA, tumor necrosis factor alpha induced protein 6 Kit ELISA, Tnfaip6 Kit ELISA, TNFAIP6 Kit ELISA

Sujet: Gene Names: TNFAIP6 TSG6
Protein names: Tumor necrosis factor-inducible gene 6 protein (Hyaluronate-binding protein) (TNF-stimulated gene 6 protein) (TSG-6) (Tumor necrosis factor alpha-induced protein 6) (TNF alpha-induced protein 6)

ID gène: 7130

UniProt: P98066