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Vitronectin Kit ELISA

VTN Reactivité: Humain Colorimetric Sandwich ELISA 0.8-200 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703572
  • Antigène Voir toutes Vitronectin (VTN) Kits ELISA
    Vitronectin (VTN)
    Reactivité
    • 3
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.8-200 ng/mL
    Seuil minimal de détection
    0.8 ng/mL
    Application
    ELISA
    Fonction
    Human Vitronectin ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF
    Sensibilité
    0.8 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples10,000 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation de l'échantillon

    For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 198 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Vitronectin (VTN) Kits ELISA
    Vitronectin (VTN)
    Autre désignation
    Vitronectin (VTN Produits)
    Synonymes
    VTN Kit ELISA, DKFZp470F0511 Kit ELISA, LOC100228142 Kit ELISA, vtn Kit ELISA, zgc:112277 Kit ELISA, V75 Kit ELISA, VN Kit ELISA, VNT Kit ELISA, AI256434 Kit ELISA, Vn Kit ELISA, Aa1018 Kit ELISA, vitronectin Kit ELISA, vitronectin a Kit ELISA, vitronectin b Kit ELISA, vitronectin L homeolog Kit ELISA, VTN Kit ELISA, vtn Kit ELISA, vtna Kit ELISA, vtnb Kit ELISA, CpipJ_CPIJ016691 Kit ELISA, vtn.L Kit ELISA, Vtn Kit ELISA
    Sujet
    Gene Names: VTN
    Protein names: Vitronectin (VN) (S-protein) (Serum-spreading factor) (V75) [Cleaved into: Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B]
    ID gène
    7448
    UniProt
    P04004
    Pathways
    Autophagy, Smooth Muscle Cell Migration
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