VWF Kit ELISA

N° du produit ABIN2703573

Détail du produit VWF Kit ELISA

Antigène: VWF (Von Willebrand Factor (VWF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.13-120 ng/mL

Seuil minimal de détection: 0.13 ng/mL

Application: ELISA

Fonction: Human VWF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human vWF. Other species not determined.

Sensibilité: 0.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour VWF Kit ELISA (ABIN2703573)

Małkiewicz, Słomiński, Skrzypkowska, Siebert, Gutknecht, Myśliwska: "The GA genotype of the -1154 G/A (rs1570360) vascular endothelial growth factor (VEGF) is protective against hypertension-related chronic kidney disease incidence." dans: Molecular and cellular biochemistry, Vol. 418, Issue 1-2, pp. 159-65, (2016) (PubMed).

Małkiewicz, Myśliwska, Skrzypkowska, Słomiński, Siebert, Gutknecht: "Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease." dans: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 38, Issue 5, pp. 469-75, (2016) (PubMed).

Małkiewicz, Skrzypkowska, Słomiński, Siebert, Gutknecht, Myśliwska: "The GG genotype of the -152 G/A vascular endothelial growth factor (VEGF) polymorphism predisposes to hypertension-related chronic kidney disease." dans: Molecular biology reports, Vol. 43, Issue 9, pp. 967-75, (2016) (PubMed).

Ma, Su, Zhang, Ling, Yin, Bai, Ruan: "The co-influence of VWD type 2B/2M mutations in the A1 domain and platelet GPIbα on the rate of cleavage to VWF by ADAMTS13." dans: Thrombosis research, Vol. 136, Issue 5, pp. 987-95, (2015) (PubMed).

Zhai, Chen, Gao, Ma, Chen, Yu, Li, Liu, Xiang, Xie, Yang: "Inhibition of the NF-κB pathway by R65 ribozyme gene via adeno-associated virus serotype 9 ameliorated oxidized LDL induced human umbilical vein endothelial cell injury." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9912-21, (2015) (PubMed).

Détail du antigène

Antigène: VWF (Von Willebrand Factor (VWF))

Autre désignation: vWF (VWF Produits)

Synonymes: VWF Kit ELISA, si:ch1073-474e24.1 Kit ELISA, F8VWF Kit ELISA, VWD Kit ELISA, 6820430P06Rik Kit ELISA, AI551257 Kit ELISA, B130011O06Rik Kit ELISA, C630030D09 Kit ELISA, von Willebrand factor Kit ELISA, Von Willebrand factor Kit ELISA, VWF Kit ELISA, vwf Kit ELISA, Vwf Kit ELISA

Sujet: Gene Names: VWF F8VWF
Protein names: von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]

ID gène: 7450

UniProt: P04275