PTPN11 Kit ELISA

N° du produit ABIN2747886

Détail du produit PTPN11 Kit ELISA

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Épitope: pTyr542

Reactivité: Rat, Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-SHP2 (Y542) ELISA Kit. This assay semi-quantitatively measures phophorylated SHP2 (Tyr542) in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse, and rat SHP2 phosphorylated at site Tyrosine-542

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Autre désignation: SHP2 (PTPN11 Produits)

Synonymes: BPTP3 Kit ELISA, CFC Kit ELISA, NS1 Kit ELISA, PTP-1D Kit ELISA, PTP2C Kit ELISA, SH-PTP2 Kit ELISA, SH-PTP3 Kit ELISA, SHP2 Kit ELISA, 2700084A17Rik Kit ELISA, AW536184 Kit ELISA, PTP1D Kit ELISA, SAP-2 Kit ELISA, SHP-2 Kit ELISA, Shp2 Kit ELISA, Syp Kit ELISA, SYP Kit ELISA, bptp3 Kit ELISA, cfc Kit ELISA, ns1 Kit ELISA, ptp-2 Kit ELISA, ptp2c Kit ELISA, ptpn11 Kit ELISA, ptpn11-a Kit ELISA, ptpn11-b Kit ELISA, shp-2 Kit ELISA, shp2 Kit ELISA, fa14b09 Kit ELISA, wu:fa14b09 Kit ELISA, wu:fi24f03 Kit ELISA, zgc:55388 Kit ELISA, zgc:63553 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 S homeolog Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, a Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, b Kit ELISA, PTPN11 Kit ELISA, Ptpn11 Kit ELISA, ptpn11.S Kit ELISA, ptpn11a Kit ELISA, ptpn11b Kit ELISA

Classe de substances: Viral Protein

Sujet: SHP2 phosphorylated at Tyrosine-542

ID gène: 5781

UniProt: Q06124

Pathways: Signalistation JAK/STAT, Signalisation RTK, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, L'effet Warburg