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SMAD1 Kit ELISA

SMAD1 Reactivité: Humain pSer463 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2747896
  • Antigène Voir toutes SMAD1 Kits ELISA
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Épitope
    pSer463, pSer465
    Reactivité
    • 10
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-SMAD1 (SER463/465) Kit. This assay semi-quantitatively measures phophorylated SMAD1 (Ser463/465) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human SMAD1 phosphorylated at site Serine-463/465.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes SMAD1 Kits ELISA
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Autre désignation
    SMAD1 (SMAD1 Produits)
    Synonymes
    MLP Kit ELISA, Xmad1 Kit ELISA, Xsmad1 Kit ELISA, bsp1 Kit ELISA, jv4-1 Kit ELISA, jv41 Kit ELISA, madh1 Kit ELISA, madr1 Kit ELISA, smad-1 Kit ELISA, MADH1 Kit ELISA, fb39h09 Kit ELISA, wu:fb39h09 Kit ELISA, wu:fb97e04 Kit ELISA, 2/23 Kit ELISA, CG12399 Kit ELISA, Dmel\\CG12399 Kit ELISA, E(zen)2 Kit ELISA, En(vvl) Kit ELISA, MAD Kit ELISA, Mat Kit ELISA, P-Mad Kit ELISA, P-mad Kit ELISA, PMad Kit ELISA, Smad Kit ELISA, Smad1 Kit ELISA, apg Kit ELISA, c28 Kit ELISA, dMAD Kit ELISA, dMad Kit ELISA, l(2)K00237 Kit ELISA, l(2)k00237 Kit ELISA, mad Kit ELISA, p-Mad Kit ELISA, pMAD Kit ELISA, pMad Kit ELISA, pSmad Kit ELISA, BSP-1 Kit ELISA, BSP1 Kit ELISA, JV4-1 Kit ELISA, JV41 Kit ELISA, MADR1 Kit ELISA, AI528653 Kit ELISA, Madh1 Kit ELISA, Madr1 Kit ELISA, Mlp1 Kit ELISA, MusMLP Kit ELISA, smad1 Kit ELISA, xmad Kit ELISA, SMAD family member 1 Kit ELISA, Mothers against dpp Kit ELISA, SMAD family member 1 L homeolog Kit ELISA, smad1 Kit ELISA, SMAD1 Kit ELISA, Mad Kit ELISA, Smad1 Kit ELISA, smad1.L Kit ELISA
    Sujet
    Mothers against decapentaplegic homolog 1 (SMAD1) phosphorylated at Serine-463/465
    ID gène
    4086
    UniProt
    Q15797
    Pathways
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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