FGF2 Kit ELISA

N° du produit ABIN2747985

Détail du produit FGF2 Kit ELISA

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2-500 pg/mL

Seuil minimal de détection: 2 pg/mL

Application: ELISA

Fonction: Rat bFGF ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rat bFGF (FGF2)

Sensibilité: 2 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour FGF2 Kit ELISA (ABIN2747985)

Zhang, Zhang, Liu, Zhang, Zhang, Tang: "Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells." dans: Biomaterials, Vol. 142, pp. 90-100, (2018) (PubMed).

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: bFGF (FGF2 Produits)

Synonymes: BFGF Kit ELISA, FGF-2 Kit ELISA, FGFB Kit ELISA, HBGF-2 Kit ELISA, Fgf-2 Kit ELISA, Fgfb Kit ELISA, bFGF Kit ELISA, fibroblast growth factor 2 Kit ELISA, fibroblast growth factor 2 (basic) Kit ELISA, FGF2 Kit ELISA, Fgf2 Kit ELISA, fgf2 Kit ELISA

Sujet: Gene Names: Fgf2 Fgf-2
Protein names: Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)

ID gène: 54250

UniProt: P13109

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins