EPH Receptor A5 Kit ELISA (EPHA5)

Details for Product EPHA5 ELISA Kit No. ABIN2748079, Fournisseur: Connectez-vous pour afficher
Antigène
  • CEK7
  • EHK-1
  • EHK1
  • EK7
  • HEK7
  • TYRO4
  • AI854630
  • AW125296
  • Cek7
  • Ehk1
  • Els1
  • Hek7
  • Rek7
  • bsk
  • Els1.
  • EPHA5
  • EPH receptor A5
  • Eph receptor A5
  • EPHA5
  • epha5
  • Epha5
Reactivité
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
5
4
2
Type de méthode
Sandwich ELISA
Application
ELISA
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Fonction Custom Rat EphA5 ELISA Kit.
Type d'échantillon Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité The antibody pair provided in this kit recognizes Rat EphA5
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others EPH Receptor A5 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation EphA5 (EPHA5 ELISA Kit Extrait)
Sujet EphA5
ID gène 79208
UniProt P54757
Pathways Signalisation RTK
Volume d'échantillon 100 μL
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Procédure de l'essai

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions For Research Use only
Stock -20 °C
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months