Galectin 3 Kit ELISA

N° du produit ABIN2748120

Détail du produit Galectin 3 Kit ELISA

Antigène: Galectin 3 (LGALS3)

Reactivité: Souris, Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 20.48-5000 pg/mL

Seuil minimal de détection: 20.48 pg/mL

Application: ELISA

Fonction: Rhesus Monkey Galectin-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Rhesus Monkey Galectin-3.

Sensibilité: 20 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples50 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Galectin 3 (LGALS3)

Autre désignation: Galectin-3 (LGALS3 Produits)

Synonymes: CBP 35 Kit ELISA, Gal-3 Kit ELISA, Galectin-3 Kit ELISA, leg3 Kit ELISA, galactoside-binding Kit ELISA, galectin-3 Kit ELISA, lectin Kit ELISA, soluble Kit ELISA, CBP35 Kit ELISA, GAL3 Kit ELISA, GALBP Kit ELISA, GALIG Kit ELISA, L31 Kit ELISA, LGALS2 Kit ELISA, MAC2 Kit ELISA, MGC52947 Kit ELISA, MGC75803 Kit ELISA, LGALS3 Kit ELISA, gal-3 Kit ELISA, GBP Kit ELISA, L-34 Kit ELISA, Mac-2 Kit ELISA, gal3 Kit ELISA, galectin 3 Kit ELISA, Galectin-3 Kit ELISA, avian beta-defensin 3 Kit ELISA, lectin, galactoside-binding, soluble, 3 L homeolog Kit ELISA, lectin, galactoside-binding, soluble, 3 Kit ELISA, galectin 2 Kit ELISA, lectin, galactose binding, soluble 3 Kit ELISA, LGALS3 Kit ELISA, leg3 Kit ELISA, AvBD3 Kit ELISA, lgals3.L Kit ELISA, lgals3 Kit ELISA, LGALS2 Kit ELISA, Lgals3 Kit ELISA

Classe de substances: Chemical

Sujet: Galectin-3 (LGALS3)

UniProt: H9FUB4

Pathways: Signalisation RTK