IL1RN Kit ELISA

N° du produit ABIN2748213

Détail du produit IL1RN Kit ELISA

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Rat Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rat Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Autre désignation: IL-1 Ra (IL1RN Produits)

Synonymes: DIRA Kit ELISA, ICIL-1RA Kit ELISA, IL-1RN Kit ELISA, IL-1ra Kit ELISA, IL-1ra3 Kit ELISA, IL1F3 Kit ELISA, IL1RA Kit ELISA, IRAP Kit ELISA, MVCD4 Kit ELISA, IRAP1 Kit ELISA, IL1RN Kit ELISA, IL-1RA Kit ELISA, F630041P17Rik Kit ELISA, interleukin 1 receptor antagonist Kit ELISA, interleukin 1 receptor antagonist-like Kit ELISA, IL1RN Kit ELISA, Il1rn Kit ELISA, IL1RNL Kit ELISA

Sujet: Interleukin 1 Receptor antagonist (IL1RN / IL-1 F3)

ID gène: 60582

UniProt: P25086

Pathways: Signalisation NF-kappaB, Hormone Transport, Cancer Immune Checkpoints