MMP 9 Kit ELISA

N° du produit ABIN2748383

Détail du produit MMP 9 Kit ELISA

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivité: Lapin

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 61-15000 pg/mL

Seuil minimal de détection: 61 pg/mL

Application: ELISA

Fonction: Rabbit MMP-9 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rabbit MMP-9

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples10 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Autre désignation: MMP-9 (MMP9 Produits)

Synonymes: CLG4B Kit ELISA, GELB Kit ELISA, MANDP2 Kit ELISA, MMP-9 Kit ELISA, AW743869 Kit ELISA, B/MMP9 Kit ELISA, Clg4b Kit ELISA, pro-MMP-9 Kit ELISA, mmp-9 Kit ELISA, fgMMP-9 Kit ELISA, mmp9 Kit ELISA, ZFMMP-9 Kit ELISA, wu:fb02g06 Kit ELISA, wu:fb07b05 Kit ELISA, wu:fi98c09 Kit ELISA, wu:fj05a08 Kit ELISA, zgc:64165 Kit ELISA, clg4b Kit ELISA, gelb Kit ELISA, mandp2 Kit ELISA, matrix metallopeptidase 9 Kit ELISA, collagenase Kit ELISA, matrix metalloproteinase 9 Kit ELISA, matrix metallopeptidase 9 S homeolog Kit ELISA, MMP9 Kit ELISA, RB3913 Kit ELISA, BPSS0666 Kit ELISA, Sbal_3732 Kit ELISA, Bcer98_0486 Kit ELISA, Shew185_0630 Kit ELISA, Shal_3056 Kit ELISA, Sbal195_0657 Kit ELISA, Lbys_3550 Kit ELISA, Palpr_2084 Kit ELISA, Mmp9 Kit ELISA, mmp9 Kit ELISA, mmp9.S Kit ELISA

Sujet: MMP-9

ID gène: 100008993

UniProt: P41246

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events